LOCUS       sequence-166785-        4356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KPNA3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KPNA3_p.V154G
FEATURES             Location/Qualifiers
     source          1..4356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2235..2334)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2351..2370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2352..2370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2375..2391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2375..2391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2822..3613
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3706..4294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4195..4214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggccgaga accccagctt ggagaaccac cgcatcaaga gcttcaagaa
      721 caagggccgc gatgtggaaa caatgcgaag acatagaaat gaagtgacag tggaactgcg
      781 gaagaacaaa agagatgaac acttattgaa aaagagaaat gttccccaag aagaaagtct
      841 agaagattca gatgttgatg ctgattttaa agcacaaaat gtaaccctag aagctatatt
      901 gcagaatgcc acaagtgata acccagtggt ccaattgagt gctgtccagg cagcaagaaa
      961 actgttatcc agtgacagaa atccaccgat tgatgactta ataaaatctg ggattttacc
     1021 aattctagtc aaatgtctag aaagggatga taatccttca ttacagtttg aagctgcttg
     1081 ggcattaact aacatagcat caggaacttc tgcacagact caagatattg ggcagtctaa
     1141 tgcagtacct ctttttctga gacttcttcg ttcaccacat cagaatgttt gtgaacaagc
     1201 agtatgggct ttgggaaaca ttataggtga tggtcctcaa tgtagagatt atgtcatatc
     1261 actgggagtt gtcaaacctc ttctgtcctt catcagtccc tccatcccca tcaccttcct
     1321 tcggaacgtc acatgggtca ttgtcaatct ctgcaggaat aaggatcccc caccgcctat
     1381 ggagacagtt caggagattt tgccagcttt atgtgtcctc atataccata cagatataaa
     1441 cattcttgta gacactgttt gggctctgtc atacttgaca gatggaggta atgaacagat
     1501 acagatggtt attgattcag gagttgtgcc ctttcttgtg ccccttctga gccatcagga
     1561 agtcaaagtt caaacagcag ccctcagagc agttggcaac atagtgactg gcaccgacga
     1621 gcagacccag gttgttctca attgtgatgt cctgtcacac ttccaaaatc tcttatcaca
     1681 cccaaaagag aagataaata aggaagcagt gtggttcctt tccaacataa cagcaggcaa
     1741 ccagcaacaa gttcaagctg taatagatgc tggattaatt cctatgataa ttcatcagct
     1801 tgctaagggg gactttggaa cacaaaaaga agctgcttgg gcaatcagca acttaacaat
     1861 aagtggcaga aaagatcagg ttgagtacct tgtacagcag gatgtaatac caccgttctg
     1921 taatttactg tcagtgaaag attctcaagt ggttcaggtg gttctagatg gtctaaaaaa
     1981 cattctgata atggccggtg atgaagcaag cacaatagct gaaataatag aggaatgtgg
     2041 aggtttggag aaaattgaag ttttacagca acatgaaaat gaagacatat ataaattagc
     2101 atttgaaatc atagatcagt atttctctgg tgatgatatt gatgaagatc cctgcctcat
     2161 tcctgaagca acacaaggag gtacctacaa ttttgatcca acagccaacc ttcaaacaaa
     2221 agaatttaat ttttacccaa ctttcttgta caaagttggc attataagaa agcattgctt
     2281 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2341 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2401 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     2461 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     2521 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     2581 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     2641 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     2701 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     2761 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2821 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2881 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2941 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     3001 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     3061 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     3121 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     3181 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     3241 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     3301 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     3361 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     3421 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     3481 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3541 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     3601 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     3661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3841 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4321 gttcctggcc ttttgctggc cttttgctca catgtt
//
