LOCUS       sequence-166913-        4812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KIF2B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KIF2B_p.E211K
FEATURES             Location/Qualifiers
     source          1..4812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2212..2311)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2328..2347)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2329..2347)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2352..2368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2352..2368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2799..3590
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3683..4271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4172..4191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4425..4442
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4601..4628)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4720..4806)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggccagc cagttctgcc tccctgaatc cccatgtctc tcgcccctga
      241 aacccttgaa gccacatttc ggagacatcc aagagggcat ctacgtggcg atccagcgca
      301 gtgacaagcg gatccacctc gctgtggtca cggagatcaa cagagaaaac tattgggtca
      361 cggtagagtg ggtggagaaa gcagtcaaaa aaggcaagaa gattgacctg gagaccatac
      421 tcctgctgaa tccagctctg gactctgctg aacaccccat gccgcccccg cccttatccc
      481 ccttggctct ggcgccctct tcggccatca gggaccagcg taccgccacg aaatgggttg
      541 cgatgatccc ccagaaaaac caaacagcct caggggacag cctggatgtg agggtcccca
      601 gcaaaccttg tctgatgaag cagaaaaagt ctccctgcct ctgggaaatc cagaaactgc
      661 aggagcagcg ggaaaagcgc aggcggctgc agcaggagat ccgagctaga cgcgccctcg
      721 atgtcaatac cagaaacccc aactacgaaa tcatgcacat gatcgaagag tatcgcaggc
      781 acctggacag cagcaagatc tcagtcctgg agcccccgca aaaacatcgc atctgcgtct
      841 gcgtgaggaa gcggcctctc aaccagcgag agacaacctt gaaggacctg gatatcatca
      901 ccgtcccctc ggacaatgtg gttatggtgc atgagtccaa gcaaaaggtg gacctcactc
      961 gctacctgca gaaccagacc ttctgcttcg accatgcctt cgatgacaaa gcctccaacg
     1021 agttggtgta ccagttcacc gcccagccac tggtggagtc catcttccgc aagggcatgg
     1081 ccacctgctt tgcctatggg cagacgggaa gtgggaagac gtacaccatg ggtggagact
     1141 tttcaggaac ggcccaagat tgttctaagg gcatttatgc tttggtggca caggatgtct
     1201 ttctcctgct cagaaactcc acatatgaga agctggacct caaagtctat gggacatttt
     1261 ttgagattta tgggggcaag gtgtatgatt tgttgaactg gaagaagaag ctgcaagtcc
     1321 ttgaggatgg caatcagcaa atccaagtgg tcgggctgca ggagaaagag gtgtgttgtg
     1381 tggaggaagt gctgaacctg gtggaaatag ggaatagctg tcggacttcc aggcaaacat
     1441 ctgtcaacgc tcactcatcc aggagccatg cagtgttcca gatcatcctg aagtcaggag
     1501 ggataatgca tggcaagttt tccctcgttg atttagctgg gaatgaaaga ggagcagata
     1561 caaccaaggc cagccggaaa aggcagctgg aaggggcaga gattaacaag agtcttctag
     1621 ccctcaaaga atgtattctg gctttgggtc agaacaagcc tcacacccca ttcagagcca
     1681 gcaaactcac actggtgctc cgggactcct ttataggcca gaactcctcc acttgcatga
     1741 ttgctaccat ctctccgggg atgacctctt gtgaaaacac tctcaacact ttaagatatg
     1801 caaacagagt aaaaaaatta aatgtagatg taaggcccta ccatcgtggc cactatccga
     1861 ttggacatga ggcaccaagg atgttaaaaa gtcacatcgg aaattcagaa atgtcccttc
     1921 agagggatga atttattaaa ataccttatg tacagagtga ggagcagaaa gagattgaag
     1981 aggttgaaac attacccact ctgttaggga aggataccac aatttcaggg aagggatcta
     2041 gccaatggtt ggaaaacatc caggagagag ctggtggagt acaccatgat attgattttt
     2101 gcattgcccg gtctttgtcc attttggagc agaaaattga tgctctgacc gagatccaaa
     2161 agaaactgaa attattacta gctgacctcc acgtgaagag caaggtagag tgcccaactt
     2221 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     2281 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     2341 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     2401 gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa
     2461 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     2521 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     2581 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     2641 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     2701 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     2761 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     2821 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2881 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     2941 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     3001 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     3061 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     3121 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     3181 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     3241 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     3301 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     3361 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     3421 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     3481 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     3541 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     3601 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     3661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3721 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3841 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3901 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3961 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4021 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4081 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4141 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4201 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4261 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4321 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4381 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4441 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4501 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4561 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     4621 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     4681 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     4741 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     4801 tatttgatgc ct
//
