LOCUS       sequence-166931-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd-generation lentiviral destination vector for gateway cloning 
            with puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pEpic
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..70
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    90..213
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(254..559)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(356..375)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaaattcaaa attttatcga tcacgagact agcctcgaga agcttgattc gagcaggaaa
       61 cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg aacgagaaac
      121 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      181 atactgtaaa acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa
      241 gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt
      301 cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat
      361 cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg
      421 ggactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg
      481 tgtcaataat atcactctgt acatccacaa acagacgata acggctctct cttttatagg
      541 tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cggcaaagga gccgttcatt
      601 tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca
      661 cgcagacgac gggcttcatt ctgcatggtt gtgcttaccg aaccggagat attgacatca
      721 tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca
      781 tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca
      841 aaaatcagcg cgcaa
//
