LOCUS       sequence-166947-        4590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing WBSCR17 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WBSCR17_p.E289K
FEATURES             Location/Qualifiers
     source          1..4590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1990..2089)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2106..2125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2107..2125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2130..2146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2130..2146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2577..3368
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3461..4049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3950..3969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4203..4220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4379..4406)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4498..4584)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggcttca ctgagaagag tcaaagtgct gttggtgttg aacttgatcg
      241 cggtagccgg cttcgtgctc ttcctggcca agtgccggcc catcgcggtg cgcagcggag
      301 acgccttcca cgagatccgg ccgcgcgccg aggtggccaa cctcagcgcg cacagcgcca
      361 gccccatcca ggatgcggtc ctgaagcgcc tgtcgctgct ggaggacatc gtgtaccggc
      421 agctgaatgg cttatccaaa tcccttgggc tcattgaagg ttatggtggg cggggtaaag
      481 ggggccttcc ggctactctt tccccggctg aagaagaaaa ggctaaggga ccccatgaga
      541 agtatggcta caattcatac ctcagtgaaa aaatttcact ggaccgttcc attccggatt
      601 atcgtcccac caagtgtaag gagctcaagt actccaagga cctgccccag atatccatca
      661 tattcatctt cgtgaacgag gccctgtcgg tgatcctgcg gtccgtgcac agtgccgtca
      721 atcacacgcc cacacacctg ctgaaggaaa tcattctggt ggatgacaac agcgacgaag
      781 aggagctgaa ggtcccccta gaggagtatg tccacaaacg ctaccccggg ctggtgaagg
      841 tggtaagaaa tcagaagagg gaaggcctga tccgcgctcg cattgagggc tggaaggtgg
      901 ctaccgggca ggtcactggc ttctttgatg cccacgtgga attcaccgct ggctgggctg
      961 agccggttct atcccgcatc caggaaaacc ggaagcgtgt gatcctcccc tccattgaca
     1021 acatcaaaca ggacaacttt gaggtgcagc ggtacaagaa ctcggcccac gggtacagct
     1081 gggagctgtg gtgcatgtac atcagccccc caaaagactg gtgggacgcc ggagaccctt
     1141 ctctccccat caggacccca gccatgatag gctgctcgtt cgtggtcaac aggaagttct
     1201 tcggtgaaat tggtcttctg gatcctggca tggatgtata cggaggagaa aatattgaac
     1261 tgggaatcaa ggtatggctc tgtgggggca gcatggaggt ccttccttgc tcacgggtgg
     1321 cccacattga gcggaagaag aagccatata atagcaacat tggcttctac accaagagga
     1381 atgctcttcg cgttgctgag gtctggatgg acgattacaa gtctcatgtg tacatagcgt
     1441 ggaacctgcc gctggagaat ccgggaattg acatcggtga tgtctccgaa agaagagcat
     1501 taaggaaaag tttaaagtgt aagaatttcc agtggtacct ggaccatgtt tacccagaaa
     1561 tgagaagata caataatacc gttgcttacg gggagcttcg caacaacaag gcaaaagacg
     1621 tctgcttgga ccaggggccg ctggagaacc acacagcaat attgtatccg tgccatggct
     1681 ggggaccaca gcttgcccgc tacaccaagg aaggcttcct gcacttgggt gccctgggga
     1741 ccaccacact cctccccgac acccgctgcc tggtggacaa ctccaagagt cggctgcccc
     1801 agctcctgga ctgcgacaag gtcaagagca gcctgtacaa gcgctggaac ttcatccaga
     1861 atggagccat catgaacaag ggcacgggac gctgcctgga ggtggagaac cggggcctgg
     1921 ctggcatcga cctcatcctc cgcagctgca caggtcagag gtggaccatt aagaactcca
     1981 tcaagtgatt gccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     2041 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     2101 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     2161 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     2221 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     2281 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     2341 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     2401 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     2461 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     2521 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     2581 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     2641 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     2701 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     2761 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     2821 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     2881 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     2941 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     3001 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     3061 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     3121 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     3181 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     3241 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     3301 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     3361 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     3421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4081 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4141 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4201 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4261 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4321 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     4381 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     4441 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     4501 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     4561 cgactgagcc tttcgtttta tttgatgcct
//
