LOCUS       sequence-166950-        4449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GALNT14 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GALNT14_p.R336Q
FEATURES             Location/Qualifiers
     source          1..4449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1849..1948)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1965..1984)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1966..1984)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1989..2005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1989..2005)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2436..3227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3320..3908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3809..3828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4062..4079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4238..4265)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4357..4443)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcggcgcgtg actcgtcggc tggttctgcc agtcttcggg gtgctctgga
      241 tcacggtgct gctgttcttc tgggtaacca agaggaagtt ggaggtgccg acgggacctg
      301 aagtgcagac ccctaagcct tcggacgctg actgggacga cctgtgggac cagtttgatg
      361 agcggcggta tctgaatgcc aaaaagtggc gcgttggtga cgacccctat aagctgtatg
      421 ctttcaacca gcgggagagt gagcggatct ccagcaatcg ggccatcccg gacactcgcc
      481 atctgagatg cacactgctg gtgtattgca cggaccttcc acccactagc atcatcatca
      541 ccttccacaa cgaggcccgc tccacgctgc tcaggaccat ccgcagtgta ttaaaccgca
      601 cccctacgca tctgatccgg gaaatcatat tagtggatga cttcagcaat gaccctgatg
      661 actgtaaaca gctcatcaaa ttgcccaagg tgaaatgctt gcgcaataat gaacggcaag
      721 gtctggtccg gtcccggatt cggggcgctg acatcgccca gggcaccact ctgactttcc
      781 tcgacagcca ctgtgaggtg aacagggact ggctccagcc tctgttgcac agggtcaaag
      841 aggactacac gcgggtggtg tgccctgtga tcgatatcat taacctggac accttcacct
      901 acatcgagtc tgcctcggag ctcagagggg ggtttgactg gagcctccac ttccagtggg
      961 agcagctctc cccagagcag aaggctcggc gcctggaccc cacggagccc atcaggactc
     1021 ctatcatagc tggagggctc ttcgtgatcg acaaagcttg gtttgattac ctggggaaat
     1081 atgatatgga catggacatc tggggtgggg agaactttga aatctccttc cgagtgtgga
     1141 tgtgcggggg cagcctagag atcgtcccct gcagccgagt ggggcacgtc ttccagaaga
     1201 agcaccccta cgttttccct gatggaaatg ccaacacgta tataaagaac accaagcgga
     1261 cagctgaagt gtggatggat gaatacaagc aatactatta cgctgcccgg ccattcgccc
     1321 tggagaggcc cttcgggaat gttgagagca gattggacct gaggaagaat ctgcgctgcc
     1381 agagcttcaa gtggtacctg gagaatatct accctgaact cagcatcccc aaggagtcct
     1441 ccatccagaa gggcaatatc cgacagagac agaagtgcct ggaatctcaa aggcagaaca
     1501 accaagaaac cccaaaccta aagttgagcc cctgtgccaa ggtcaaaggc gaagatgcaa
     1561 agtcccaggt atgggccttc acatacaccc agcagatcct ccaggaggag ctgtgcctgt
     1621 cagtcatcac cttgttccct ggcgccccag tggttcttgt cctttgcaag aatggagatg
     1681 accgacagca atggaccaaa actggttccc acatcgagca catagcatcc cacctctgcc
     1741 tcgatacaga tatgttcggt gatggcaccg agaacggcaa ggaaatcgtc gtcaacccat
     1801 gtgagtcctc actcatgagc cagcactggg acatggtgag ctcttgattg ccaactttct
     1861 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1921 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     1981 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     2041 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     2101 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     2161 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     2221 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     2281 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     2341 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     2401 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     2461 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2521 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2581 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2641 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2701 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2761 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2821 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2881 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2941 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     3001 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     3061 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     3121 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     3181 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     3241 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3301 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3361 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3421 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3481 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3541 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3601 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3661 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3721 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3781 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3841 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3901 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3961 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4021 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4081 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4141 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     4201 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     4261 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     4321 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4381 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4441 ttgatgcct
//
