LOCUS       sequence-167013-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and
            putidaredoxin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pR1G108CP20X
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttgtcgacgg agctcgaatt cggatcgaat tctcaccact gacggtccgg
      121 aacgtcaaca acgataccgt ccagctccgg ggtcatgatg atctggcaag acagacgaga
      181 gttcggtttc agttccgcgg taacagattc cagcataccg atttcacgtt cgttagcagc
      241 cggaactttg tcggtgaacg cttcgttcac gtacacgtgg caggtcgcgc aagacgcaga
      301 accaccgcag tcaccaacga tgtcgtagat gccgttagaa actgccgcct gcatcagaga
      361 aacaccgtcc gcaacgtcca gttcgcgacg ggtaccgtcg tgagaaacgt aaacaacttt
      421 agacatagag ccaccacctc cgggcggcgg tggaggtgga ggcggcggtg gtggcggtgg
      481 aggaggcggt ggaggtggag gggaaccgcc acctcccagg cgcggcgcaa tccagaagat
      541 catgtgaccg ccgctaactg cttccacatc aattttcatc ggcagatttt caccgaaaga
      601 caccatcgtc ggcgcactaa aaccacgcag gcctttcacc gcatctttga acatttctgc
      661 gctgtagcta ctagaggcag aggtatcgat gctcagttct ttcagcggtt tatctttcat
      721 cagaaaggcc acatagcgtt taccttcttc gcccgcttca attttgattt tatcttcttc
      781 cgtgctgata cgcatttctt caccaaccag ggtcacatcg gccgcgataa cgttcagcac
      841 gctttcatcc gtggtaaaat taacggccag gttcactttc ggttccgtca gctgttcaac
      901 ctggcctttt tctgctttga tgtagatggt acttttggcg caagattttt catcacggat
      961 ga
//
