LOCUS       sequence-167089-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with V2-MESA ectodomain, 35 extracellular linkers,
            a flag tag, M cleavage sequence, and tTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V2-MESA-35F-M-tTA
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     809..830
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acctttaata gaaattggac agcaagaaag cgagcttagt gatacttgtg ggccagggca
       61 ttagccacac cagccaccac tttctgatag gcagcctgca ctggtggggt gaattgctcg
      121 aggtttaaac agctccaccg cggtggcggc cgctattact tgtacagctc gtccatgccg
      181 agagtgatcc cggcggcggt gcggaactcc agcaggacca tgtgatcgcg cttctcgttg
      241 gggtctttgc tcagcacgga ctgggtgctc aggtagtggc tgtcgggcag cagcacgggg
      301 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcc
      361 acgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgac ggccatgata
      421 tagatgttgt ggctgttgaa gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      481 ttgaagtcga cgcccttcag ctcgatgcgg ttcactaggg tgtcgccctc gaacttcacc
      541 tcggcgcggg tcttgtaggt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      601 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
      661 aagcactgca cgccgtggct cagggtggtc acgagggtgg gccagggcac gggcagcttg
      721 ccggtggtgc agatgaactt cagggtcagc ttgccgttgg tggcatcgcc ctcgccctcg
      781 cccctcacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      841 accccggtga acagctcctc gccc
//
