LOCUS       sequence-167128-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Host vector to fuse a gene at the C-terminus of PCNA1 variant 
            (G108C) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p1G108C-GG
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(860..877)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(887..904)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             923..945
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    960..984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(984..1003)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(985..1003)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatccgg tctcactccc aggcgcggcg
      121 caatccagaa gatcatgtga ccgccgctaa ctgcttccac atcaattttc atcggcagat
      181 tttcaccgaa agacaccatc gtcggcgcac taaaaccacg caggcctttc accgcatctt
      241 tgaacatttc tgcgctgtag ctactagagg cagaggtatc gatgctcagt tctttcagcg
      301 gtttatcttt catcagaaag gccacatagc gtttaccttc ttcgcccgct tcaattttga
      361 ttttatcttc ttccgtgctg atacgcattt cttcaccaac cagggtcaca tcggccgcga
      421 taacgttcag cacgctttca tccgtggtaa aattaacggc caggttcact ttcggttccg
      481 tcagctgttc aacctggcct ttttctgctt tgatgtagat ggtacttttg gcgcaagatt
      541 tttcatcacg gatgatgatt ttcaggccag aatccgtttc ggtcagttca atcgtcgctt
      601 ttttgctact tgctttgctc agaattttct tcacagagct cacatccagt ttcacagagg
      661 tcgggctatc aatactgtat tcagacagaa catctttcgg aatgcgcatg attgccatca
      721 gcactttatc ttccgtcaga tgacgactga agatgccatc ttcggtgaag ttcaggatga
      781 tagaatccgt aacgttggta atgctgttga tgaagctaaa gaaatctttg gcgttcggat
      841 acacaatttt aaacatatgg ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat
      901 gatggctgct gcccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg
      961 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
     1021 tctcgatcct ctacgccgga cgcat
//
