LOCUS       sequence-16715-e        1394 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3613
FEATURES             Location/Qualifiers
     source          1..1394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             470..490
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(533..561)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 antgntctta ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaan
       61 cactcacaac gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg
      121 tcgactctag aggatcccca attcttgaga cttccactat cttctctggc accctccact
      181 acgcgtaggc gtctctcgct tcgtgtattc ccgggaagcc gggttcccgt ctctcccgcc
      241 gctgccgctg ccgcacacag ctttacacct cgtagaatcc ccaaagaggg gcgtggcttg
      301 cgggtgccaa catcctcctg ccgaggaaga agcaggcact catcactcgc atcatcaacc
      361 tcgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca
      421 ttttcaggag gacccttgga gggtaccgag ctcagaaaaa atgactgctc caaagaagaa
      481 gcgtaaggta ccggtagaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc
      541 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      601 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      661 acctgttcca tgggtaagtt taaacatata tatactaact aaccctgatt atttaaattt
      721 tcagccaaca cttgtcacta ctttcactta tggtgttcaa tgcttctcga gatacccaga
      781 tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      841 aactatattt ttcaaagatg acgggaacta caagacacgt aagtttaaac agttcggtac
      901 taactaacca tacatatttt aaattttcag gtgctgaagt caagttttga aggtgatacc
      961 cttggttaat agaatcgagt ttaaaaggta ttgattttaa agaaaatggn aacnttctng
     1021 gacccaattg gaatacaact atnactcnca caatgnatac ntcnctgcna aaaaaaaaan
     1081 anngggatca aagcggnagt ttaacntgat tttnnaacta cctaatctga tttaattttc
     1141 cnaactncaa aattaaccca cnttganang gagcgtncct ngcgaccttt ncnaaaattc
     1201 tccatgggga gggcnntntt tnaaaaancn tncnnnccaa nttgcnttta aaaaccaaaa
     1261 aaaaacnagg gccttngatt naacccnggg aanccggngg ggaataaaan ctccaaatgg
     1321 ttattncccn aancgccntt attnaaanag agngcccctn annttgggna aangggccnc
     1381 aaaattaaat taaa
//
