LOCUS       sequence-167249-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin 
            resistance from EF-1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, 
            Koh HC, Nam JW, Kim H
  TITLE     In vivo high-throughput profiling of CRISPR-Cpf1 activity.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104.
  PUBMED    27992409
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104."
COMMENT     Sequence Label: Lenti_gRNA-Puro
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    298..415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(575..823)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(633..652)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(803..823)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg
       61 ccaccttctc taggcaccgg atcaattgcc gacccctccc cccaacttct cggggactgt
      121 gggcgatgtg cgctctgccc actgacgggc accggagcca attcccactc ctttcaagac
      181 ctagaaggtc cattagctgc aaagattcct ctctgtttaa aactttatcc atctttgcac
      241 ccgggccccc tcgagccggc gccaaagtgg atctctgctg tccctgtaat aaacccgaaa
      301 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
      361 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
      421 gatgaatact gccatttgtc tcaagatcta gttacgccaa gcttcatccc atgtaagcat
      481 gtacggtcta gaacgtgtca cgtgcacgtt aagtcgagta agctgaccgc tgaagtacaa
      541 gtggtagagt agcgagacga tagtcaacgt ctccggtgtt tcgtcctttc cacaagatat
      601 ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa
      661 aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt
      721 gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta acagccttgt
      781 atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctctctgggt cccctcgggg
      841 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
      901 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
      961 ttatatacca cagccaattt gttatgttaa accaat
//
