LOCUS       sequence-167289-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with 
            pK170..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T
  TITLE     Supernova: A Versatile Vector System for Single-Cell Labeling and 
            Gene Function Studies in vivo.
  JOURNAL   Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
  PUBMED    27775045
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-10-24"; volume: "6:35747. doi"; pages: " 
            10.1038/srep35747"
COMMENT     Sequence Label: pK168.AAV-EF1-alpha-DIO-tTA-P2A-RFP-WPRE (Supernova)
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(65..98)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(149..182)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             join(195..812,813..818,819..854,855..857,858..893,894..896,
                     897..932,933..938)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             954..1010
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attcataact tcgtatagca tacattatac gaagttattc tttgcaccat tctaaagaat
      121 aacagtgata atttctgggt taaggcaaat aacttcgtat aggatacttt atacgaagtt
      181 atgctagcgc caccatgtct agactggaca agagcaaagt cataaactct gctctggaat
      241 tactcaatga agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
      301 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgg
      361 caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc
      421 aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg
      481 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
      541 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
      601 ccgtgggcca ctttacactg ggctgcgtat tggaggatca ggagcatcaa gtagcaaaag
      661 aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc
      721 tgttcgacca tcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
      781 gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg
      841 acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg
      901 acgctcttga cgattttgac cttgacatgc tccccggggg atccggaagc ggagctacta
      961 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct atgagcgagc
     1021 tgatcaagga gaacatgcac atgaagctgt acatggaggg c
//
