LOCUS       sequence-167343-        8836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bandpass temperature-gated expression of mWasabi and mCherry 
            reporters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Piraner DI, Abedi MH, Moser BA, Lee-Gosselin A, Shapiro MG
  TITLE     Tunable thermal bioswitches for in vivo control of microbial 
            therapeutics.
  JOURNAL   Nat Chem Biol. 2017 Jan;13(1):75-80. doi: 10.1038/nchembio.2233. 
            Epub 2016 Nov 14.
  PUBMED    27842069
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2233"; journalName: "Nat Chem Biol"; date: 
            "2017-01"; volume: "13"; issue: "1"; pages: "75-80"
COMMENT     Sequence Label: pThermeleon
FEATURES             Location/Qualifiers
     source          1..8836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1281..1298
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1311..2024
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYKG"
     CDS             2071..2784
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             2797..2841
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2893..2940
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2977..3432
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3064..3083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3274..3295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(3547..4338,4339..4407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4170..4189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4408..4500)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4581..5169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5070..5089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5323..5340
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5355..5497
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5423..5442)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5583..5605
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5599..5790)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     RBS             6440..6448
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    6698..6712
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             6798..6815
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6828..7538
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6973..6991)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7241..7260
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        7592..7668
                     /gene="lacI"
                     /label=lacI promoter
     CDS             7693..8403
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="STKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQS
                     GVGALFNGINALNAYNAASLTRILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEYP
                     VFSHVQAGMLSPELRTFTKGGAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDG
                     MLILVDPEQAVEPGDFCIARLGGGEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVV
                     GKVIASQWPEETFG"
     CDS             8416..8460
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      8512..8559
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     8776..8795
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaatttta atttgtttgt
       61 tagttagttt atttgttggt ttgtttgtgt tataatatcc tctagaaata attttgttta
      121 actttaagaa ggagatatac catgcgtccg gcgacatacg aaccagaaca gattattgaa
      181 gcagggctgg ccctgcaggc tgaaggacgg aatatcaccg ggttcgcact acgtaaccag
      241 gtgggtggcg gcaatccgac acgtctccgc cagatatggg acgaatacca ggcttcacag
      301 agcacggtcg tcactgaacc cgttgccgag ctgccagtgg aagtggctga agaagtgaag
      361 gccgtctccg ccgcgctgtc cgaacgcatc acccagctgg cgacagaact gaatgacaag
      421 gcggtccggg ctgcagaacg ccgggttgcg gaagtcacgc gtgctgccgg tgaacagacc
      481 gcacaggcag agcgggagct ggccgacgcc gcgcagacag tcgacgacct ggaagaaaaa
      541 ctggatgaac tgcaggacag atatgacagt ttgacgctgg cgctggagtc agaacgttca
      601 ctgcgtcagc agcatgatgt ggagatggcc cagctgaaag agcgtcttgc ggccgctgaa
      661 gagaataccc gtcagcgaga ggaacggtat caggagcaga agacagtgct gcaggatgcg
      721 cttaatgcgg agcaggcaca gcacaaaaac acgcgggaag acctgcagaa acgactggag
      781 caaatttctg ccgaagctaa tgcgcgtaca gaagaactga agtctgaacg cgataaagtc
      841 aatactctcc ttacccgcct tgaatcgcag gaaaatgcgc tggcctcaga acgtcagcag
      901 catctggcca cccgcgaaac gctgcagcaa cgcctcgagc aggccatcgc tgacacgcag
      961 gcgcgcgccg gtgagattgc acttgaacgt gacagagtca gcagcctcac cgcaaggctg
     1021 gaatcgcagg aaaaggcctc ctcggagcaa ctggtgcgta tgggcagtga aatagccagt
     1081 ctgacagagc gttgcacaca gctggaaaac cagcgtgatg atgcccgtct ggagacgatg
     1141 ggggagaaag aaacggtcgc ggcactgcgt ggtgaggctg aagccctgaa gcgtcagaac
     1201 cagtcactga tggcggcgct ttcaggcaat aaacagaccg gtggccagaa tgcgtgagga
     1261 ggacaatcat gggcagcagc catcaccatc atcaccacag ccaggatcca atggtgagca
     1321 agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag ctgaagatgg
     1381 agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc aagccctacg
     1441 acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc ttctcctacg
     1501 acattctgac caccgcgttc agttacggca acagggcctt caccaagtac cccgacgaca
     1561 tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc accatgacct
     1621 tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca
     1681 tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaagg
     1741 agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg
     1801 gcgacgtcaa gatgaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga
     1861 ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca
     1921 tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag atcgccgtgg
     1981 cccgcaactc caccgacggc atggacgagc tgtacaaggg ctgacgcaaa aaaccccgct
     2041 tcggcggggt tttttcgcca ccatacactg atgagcacaa aaaagaaacc attaacacaa
     2101 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt
     2161 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct
     2221 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc
     2281 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa
     2341 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt
     2401 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg
     2461 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc
     2521 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac
     2581 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt
     2641 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag
     2701 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag
     2761 tggcctgaag agacgtttgg ctgactcgag tctggtaaag aaaccgctgc tgcgaaattt
     2821 gaacgccagc acatggactc gtctactagc gcagcttaat taacctaggc tgctgccacc
     2881 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     2941 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
     3001 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3061 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3421 cgtttacaat ttctggcggc acgatggcat gagattatca aaaaggatct tcacctagat
     3481 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3541 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3601 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3661 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3721 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3781 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3841 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3901 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3961 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4021 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4081 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4141 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4201 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4261 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4321 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4381 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatcatgat tgaagcattt
     4441 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4501 taggtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5281 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5341 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     5401 cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     5461 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     5521 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5581 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     5641 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     5701 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     5761 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     5821 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     5881 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     5941 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     6001 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     6061 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     6121 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     6181 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     6241 gatcatgcta gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     6301 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
     6361 ctatcaccgc aagggataaa tatctaacac cgtgcgtgtt gactatttta cctctggcgg
     6421 tgataatggt tgcatgtact aaggaggttg tatggaacaa cgcataaccc tgaaagatta
     6481 tgcaatgcgc tttgggcaaa ccaagacagc taaaagatct ctcacctacc aaacaatgcc
     6541 cccctgcaaa aaataaattc atataaaaaa catacagata accatctgcg gtgataaatt
     6601 atctctggcg gtgttgacat aaataccact ggcggtgata ctgagcacat cagcaggacg
     6661 cactgaccac catgaaggtg acgctcttaa aaattaagcc ctgaagaagg gcagcattca
     6721 aagcagaagg ctttggggtg tgtgatacga aacgaagcat tggttaaaaa ttaaggaggg
     6781 aattcatggg cagcagccat caccatcatc accacagcca ggatccaatg gtgagcaagg
     6841 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     6901 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     6961 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     7021 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     7081 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     7141 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     7201 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     7261 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     7321 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     7381 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     7441 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     7501 gccactccac cggcggcatg gacgagctgt acaagtaaaa gcttgcggcc gcataatgct
     7561 taagtcgaac agaaagtaat cgtatgagct cgacaccatc gaatggcgca aacctttcgc
     7621 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt ataagaagga
     7681 gatatacatg tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt
     7741 cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc
     7801 gcagacaaga tggggatggg gcagtcaggc gttggtgcct tatttaatgg catcaatgca
     7861 ttaaatgctt ataacgccgc atcgcttaca agaattctca aagttagcgt tgaagaattt
     7921 agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca
     7981 cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat gctctcacct
     8041 gagcttagaa cctttaccaa aggtggtgcg gaaaggtggg taagcacaac caaaaaagcc
     8101 agtgattctg cattctggct tgaggttgaa ggtaattcca tgacagcacc aacaggctcc
     8161 aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca
     8221 ggtgatttct gcatagccag actcgggggt ggtgagttta ccttcaagaa actgatcagg
     8281 gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat
     8341 gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc
     8401 tgactcaagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg
     8461 tctactagcg cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa
     8521 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg cgggatctcg
     8581 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     8641 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     8701 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     8761 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     8821 ggtgatgccg gccacg
//
