LOCUS       sequence-167420-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mScarlet-H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pmScarlet-H_C1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..737
                     /codon_start=1
                     /product="monomeric red fluorescent protein evolved from a 
                     synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-H
                     /note="more photostable than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     HALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(175..193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     443..462
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    738..803
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg
       61 cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg aacggccacg
      121 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      181 tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc cctcagttca
      241 tgtacggctc cagggccttc accaagcacc ccgccgacat ccccgactac tataagcagt
      301 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgccgtga
      361 ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg aagctccgcg
      421 gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc tgggaagcgt
      481 ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag cacgccctgc
      541 gcctgaagga cggcggccgc tacctggcgg acttcaagac cacctacaag gccaagaagc
      601 ccgtgcagat gcccggcgcc tacaacgtcg accgcaagtt ggacatcacc tcccacaacg
      661 aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc accggcggca
      721 tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
      781 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
      841 catttgtaga ggttt
//
