LOCUS       sequence-167474-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR 
            of the queC gene from B. subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(249..266)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(249..265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(258..280)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(474..493)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     593..615
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(653..671)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        739..843
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttacgaattc gcgagagaca tcgctacagc taattcacgc aaaaaaacac cttttttcgg
       61 aaggtgctct agaaaaacat aaaaaaggcc gtatccaagg atacagctcg tccttagttt
      121 tttatagagg gtgtagctag aacctctccc gcatatcgca cggtaccgaa gcaattatac
      181 tgtatgctca tacagtatca agtgttttgg gatcctctag agtcgacctg caggcatgca
      241 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      301 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      421 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      481 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      541 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      601 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      661 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      721 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      781 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      841 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      901 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      961 gcacgagtgg gttacat
//
