LOCUS       sequence-167480-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli RecA promoter followed by stretch with no A's followed by 
            riboswitch for single-round transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(256..273)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(256..272)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(265..287)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(481..500)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     600..622
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(660..678)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        746..850
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aattcgcgag agacatcgct acagctaatt cacgcaaaaa aacacctttt ttcggaaggt
       61 gctctagaaa aacataaaaa aggccgtatc caaggataca gctcgtcctt agttttttat
      121 agagggtgta gctagaacct ctcccgcata tcgcacggaa cgcagagcgg taccgaagca
      181 attatactgt atgctcatac agtatcaagt gttttgggat cctctagagt cgacctgcag
      241 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      301 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      361 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
      421 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      481 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
      541 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      601 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
      661 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      721 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      781 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      841 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      901 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      961 gttgggtgca cgagtgggtt acatcgaact gg
//
