LOCUS       sequence-167486-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pVR2 but with a C to U mutation in the riboswitch aptamer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Widom JR, Nedialkov YA, Rai V, Hayes RL, Brooks CL 3rd, Artsimovitch
            I, Walter NG
  TITLE     Ligand Modulates Cross-Coupling between Riboswitch Folding and 
            Transcriptional Pausing.
  JOURNAL   Mol Cell. 2018 Nov 1;72(3):541-552.e6. doi: 
            10.1016/j.molcel.2018.08.046.
  PUBMED    30388413
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08"; journalName: "Mol Cell"; date: 
            "2018-11-1- 1"; volume: "72"; issue: "3"; pages: "541-552.e6"
COMMENT     Sequence Label: pVR2-m1
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(261..278)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(261..277)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(270..292)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(486..505)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     605..627
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(665..683)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        751..855
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttacgaattc gcgagagaca tcgctacagc taattcacgc aaaaaaacac cttttttcgg
       61 aaggtgctct agaaaaacat aaaaaaggcc gtatccaagg atacagctcg tccttagttt
      121 tttatagagg gtgtagctaa aacctctccc gcatatcgca cggaacgcag agcggtaccg
      181 aagcaattat actgtatgct catacagtat caagtgtttt gggatcctct agagtcgacc
      241 tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      301 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      361 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      421 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      481 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      541 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
      601 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
      661 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      721 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      781 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      841 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      901 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      961 gatcagttgg g
//
