LOCUS       sequence-167601-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA781  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM781
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        156..501
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     444..464
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    662..838
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1400..1418)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1401..1418)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2057..2076
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2179..2184,2185..2201,2202..2207))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2482..2862
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2868..3419
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3429..3681
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3767..3791
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4216..5010
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5097..5685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5839..5856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5871..6011
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5937..5956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6097..6119
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6354..6548
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6614..7687)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8116..8745)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10046..10070
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10258..10280
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10272..10289
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10273..10289
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10314..11021)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11200..11219
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11200..11218
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccacgagga gcatcgtggg aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
       61 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat
      121 gtcaaagata ctgtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt
      181 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acctcatcga aaggacagta
      241 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa
      301 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      361 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac
      421 ataagggatg acgcacaacc ccactatcct tcgcaagacc cttcctctat ataaggaagt
      481 tcatttcatt tggagaggac agcccaagct ttaagtatcc agctaaaaac tctaagttgt
      541 tgttgttatg gtctaattta aatatggtct aaagaagaag aattaagtat ccagctaaaa
      601 actctaagaa ttcggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta
      661 ttttctccat aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg
      721 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
      781 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatctc
      841 ctaaagtccc tatagatctt tgtcgtgaat ataaactaga cacgagacga ctagacctgg
      901 agcccagacg ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt agggaaaaga
      961 tgctaaggca gggttggtta cgttgactcc cccgtaggtt tggtttaaat atgatgaagt
     1021 ggacggaagg aaggaggaag acaaggaagg ataaggttgc aggccctgtg caaggtaaga
     1081 agatggaaat ttgatagagg tacgctacta tacttatact atacgctagg ggaatgattg
     1141 tatttatacc ctataccccc taataacccc ttatcaattt aagaaataat ccgcataagc
     1201 ccccgcttaa aaattggtat cagagccatg aataggtcta tgaccaaaac tcaagaggat
     1261 aaaacctcac caaaatacga aagagtcctt aactctaaag ataaaagatc tttcaagatc
     1321 aaaactagtt ccctcacacc ggtgacgggg atcgcatgcg acctctagac catcacattg
     1381 gagcttcagc tgctcgagtt ctatagtgtc acctaaatcg tatgtgtatg acacataagg
     1441 ttatgtatta attgtagccg cgttcttacg tcgacgtacc aattacgccc cgccctgtca
     1501 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1561 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     1621 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     1681 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     1741 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     1801 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     1861 aacggtgtaa caagggtgaa cactatccca tattaccagc tcaccgtctt tcattgccat
     1921 acggaactcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     1981 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     2041 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     2101 ggatatatca acggtggtat atccagtgat ttctttctcc attttagctt ccttagctcc
     2161 tgaaaatctc gataactcat taaagcttat cgatgataag ctgtcaaaca tgagaattta
     2221 gcccgcctaa tgagcgggct ttttgttgtg atccgcgttg ctggcgtttt tccataggct
     2281 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2341 aggactataa agataccagg cgtttccccc ctggaagctc cctcgtgcgc tctcctgttc
     2401 cgaccctgcc gcttaccgga tacctgtccg cctttcttaa ttaactctct agactcacct
     2461 aggatccatg aattctgaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatccga
     2521 gtcagttttt atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc
     2581 gggcctgaat ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt
     2641 aacgactttt gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac
     2701 aacgctttac agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt
     2761 cagaaatgga taaatagcct tgcttcctat tatatcttcc caaattacca atacattaca
     2821 ctagcatctg aatttcataa ccaatctcga tacaccaaat cgaatcgatg agcccagaac
     2881 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg
     2941 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg
     3001 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg
     3061 acggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact
     3121 ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca
     3181 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg
     3241 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc
     3301 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc
     3361 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatttgac
     3421 tgcagaatct tggactccca tgttggcaaa ggcaaccaaa caaacaatga atgatccgct
     3481 cctgcatatg gggcggtttg agtatttcaa ctgccatttg ggctgaattg aagacatgct
     3541 cctgtcagaa attccgtgat cttactcaat attcagtaat ctcggccaat atcctaaatg
     3601 tgcgtggctt tatctgtctt tgtattgttt catcaattca tgtaacgttt gcttttctta
     3661 tgaattttca aataaattat cagatccgag ctcgaattaa ttcggcgtta attcagtaca
     3721 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     3781 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     3841 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     3901 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     3961 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     4021 ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga
     4081 aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga
     4141 attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga
     4201 ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
     4261 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
     4321 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
     4381 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
     4441 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
     4501 gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat
     4561 gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc
     4621 ttagacagcc gcttagccga attggattac ttactgaata acgatctggc cgatgtggat
     4681 tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta
     4741 aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac
     4801 atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg
     4861 gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa
     4921 cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa
     4981 tattatattt tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa
     5041 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5101 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5161 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5221 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5281 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5341 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5401 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5461 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5521 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5581 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5641 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5701 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5761 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5821 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     5881 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     5941 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     6001 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6061 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6121 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     6181 agtggcgacg gcgcggcttg tccgcgccct ggagattgcc tggccgtagg ccagccattt
     6241 ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg
     6301 accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac
     6361 aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc
     6421 cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg
     6481 ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc
     6541 tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca
     6601 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
     6661 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
     6721 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
     6781 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
     6841 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
     6901 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
     6961 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
     7021 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
     7081 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
     7141 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
     7201 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
     7261 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
     7321 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
     7381 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
     7441 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
     7501 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
     7561 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
     7621 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
     7681 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac
     7741 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
     7801 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
     7861 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
     7921 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
     7981 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
     8041 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
     8101 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
     8161 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
     8221 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
     8281 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
     8341 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
     8401 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
     8461 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
     8521 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
     8581 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
     8641 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
     8701 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
     8761 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
     8821 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
     8881 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
     8941 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
     9001 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
     9061 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
     9121 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
     9181 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
     9241 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
     9301 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
     9361 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
     9421 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
     9481 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
     9541 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
     9601 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
     9661 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
     9721 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
     9781 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
     9841 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
     9901 tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc
     9961 atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat
    10021 tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata
    10081 gtttaaactg aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc
    10141 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    10201 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    10261 agtcacgacg ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcctgctga
    10321 gcctcgacat gttgtcgcaa aattcgccct ggacccgccc aacgatttgt cgtcactgtc
    10381 aaggtttgac ctgcacttca tttggggccc acatacacca aaaaaatgct gcataattct
    10441 cggggcagca agtcggttac ccggccgccg tgctggaccg ggttgaatgg tgcccgtaac
    10501 tttcggtaga gcggacggcc aatactcaac ttcaaggaat ctcacccatg cgcgccggcg
    10561 gggaaccgga gttcccttca gtgagcgtta ttagttcgcc gctcggtgtg tcgtagatac
    10621 tagcccctgg ggcacttttg aaatttgaat aagatttatg taatcagtct tttaggtttg
    10681 accggttctg ccgctttttt taaaattgga tttgtaataa taaaacgcaa ttgtttgtta
    10741 ttgtggcgct ctatcataga tgtcgctata aacctattca gcacaatata ttgttttcat
    10801 tttaatattg tacatataag tagtagggta caatcagtaa attgaacgga gaatattatt
    10861 cataaaaata cgatagtaac gggtgatata ttcattagaa tgaaccgaaa ccggcggtaa
    10921 ggatctgagc tacacatgct caggtttttt acaacgtgca caacagaatt gaaagcaaat
    10981 atcatgcgat cataggcgtc tcgcatatct cattaaagca ggactctagg gactagtccc
    11041 gggtcttaat taagtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaaggaga
    11101 tcctttggat cacaaaaaaa aagcccgctc attaggcggg ttaaattccc gggtactgca
    11161 gccgattcat taatgcaggt taacctggct tatcgaaatt aatacgactc actataggga
    11221 gaccggcaga tctgatccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag
    11281 ggctattgag acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc
    11341 agctatctgt cacttcatcg aaaggacagt ggaaaaggaa ggtggctcct acaaatgcca
    11401 tcattgcgat aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga
    11461 tggaccccca
//
