LOCUS       sequence-16764-e        1414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4053
FEATURES             Location/Qualifiers
     source          1..1414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             630..650
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             672..692
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             714..734
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             756..776
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(819..847)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttacatgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
       61 aatcactcac aacgatggat acgctaacaa cttggaaatg aaataagctt gcatgcctgc
      121 aggccttggt cgactctaga ggatccccgg ggattggcca aaggacccaa aggtatgttt
      181 cgaatgatac taacataaca tagaacattt tcaggaggac ccttgcttgg ctagcaaaaa
      241 tgcataaggt tttgctggca ctgttcttta tctttctggc accagcatcc gcactggcag
      301 tctccgaacc ggcctgcagg tcgatttttt gcaaattacg agcgttgtag ggggcggagc
      361 gataggtcct ataggttttg gtatatcatc attcattcat tcattggtac attcatttac
      421 ccaccttcct ctttctgagc ttctctggag ttctgtgctt cctttttccc ttatctttat
      481 actgtaattt ttaactttca ggcattgatt ggatccccgg gattggccaa aggacccaaa
      541 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgcttgga
      601 gggtaccgag ctcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggcagaaa
      661 aaatgactgc tccaaagaag aagcgtaagg taccggcaga aaaaatgact gctccaaaga
      721 agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
      781 tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      841 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      901 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg
      961 taagttaaac atatatatac taactaaccc tgattanttt aaattttcag ccaacactgg
     1021 tcactacttt ctgttatggg gttcaangct tcncgagana cccanatcat atgaaacggc
     1081 atgacttttt caaaagngcc aggccnaang tnangtacag gaaaaaatan ntttttcaaa
     1141 atgacgggaa ctaacananc nnnannttaa acgntcggac tactaaccaa caatttaant
     1201 tncngggctn ancnagttga agggaancct gntaanaatc cantaaaggn ntgatttaaa
     1261 aangnaaaat nngggancnt tggaaacntt aaccnccatg ttcccngggg aanccgggan
     1321 gaaaaagggg gntaaggggt ttaaanattt cnannnttnc gattttttaa ccccnaaagg
     1381 gggngnngnn tttnanaaac ttggaacccg tggg
//
