LOCUS       sequence-167668-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the mitochondria (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pMTS_mScarlet-H_N1
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             85..159
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      187..196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             193..888
                     /codon_start=1
                     /product="monomeric red fluorescent protein evolved from a 
                     synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-H
                     /note="more photostable than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     HALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(326..344)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     594..613
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    889..954
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcatgtcc gtcctgacgc cgctgctgct gcggggcttg
      121 acaggctcgg cccggcggct cccagtgccg cgcgccaaga tccattcgtt gggggatcca
      181 ccggtcgcca ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag
      241 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      301 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      361 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt caccaagcac
      421 cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc
      481 gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac
      541 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
      601 atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc
      661 gtgctgaagg gcgacattaa gcacgccctg cgcctgaagg acggcggccg ctacctggcg
      721 gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc
      781 gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa
      841 cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
      901 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atcc
//
