LOCUS       sequence-167705-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the ER (can be used for colocalization 
            studies and the OSER assay).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pCytERM_mScarlet-i_N1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      224..233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(363..381)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     631..650
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctgg taccccttgc
       61 agcttccagt tgtcaggaag agaaggctcc catggatcct gtggtggtgc tggggctctg
      121 tctctcctgt ttgcttctcc tttcactctg gaaacagagc tatgggggag ggaagcttcg
      181 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa
      241 gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa
      301 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      361 cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc
      421 tcagttcatg tacggctcca gggccttcat caagcacccc gccgacatcc ccgactacta
      481 taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      541 cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa
      601 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg
      661 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat
      721 ggccctgcgc ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc
      781 caagaagccc gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc
      841 ccacaacgag gactacaccg tgg
//
