LOCUS       sequence-167725-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of NudB (E. coli) and PMD (S. cerevisiae) are under 
            control of Trc promoter for isopentenol production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   George KW, Thompson MG, Kang A, Baidoo E, Wang G, Chan LJ, Adams PD,
            Petzold CJ, Keasling JD, Lee TS
  TITLE     Metabolic engineering for the high-yield production of 
            isoprenoid-based C(5) alcohols in E. coli.
  JOURNAL   Sci Rep. 2015 Jun 8;5:11128. doi: 10.1038/srep11128.
  PUBMED    26052683
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-8"; volume: "5:11128. doi"; pages: " 
            10.1038/srep11128"
COMMENT     Sequence Label: JBEI-6833
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(959..970)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gccaagcttg catgcctgca ggtcgactct agaggatccc ctcccgggtt attcctttgg
       61 tagaccagtc tttgcgtcaa tcaaagattc gtttgtttct tgtgggcctg aaccgacttg
      121 agttaaaatc actctggcaa catccttttg caactcaaga tccaattcac gtgcagtaaa
      181 gttagatgat tcaaattgat ggttgaaagc ctcaagctgc tcagtagtaa atttcttgtc
      241 ccatccagga acagagccaa acaatttata gataaatgca aagagtttcg actcattttc
      301 agctaagtag tacaacacag catttggacc tgcatcaaac gtgtatgcaa cgattgtttc
      361 tccgtaaaac tgattaatgg tgtggcacca actgatgata cgcttggaag tgtcattcat
      421 gtagaatatt ggagggaaag agtccaaaca tgtggcatgg aaagagttgg aatccatcat
      481 tgtttccttt gcaaaggtgg cgaaatcttt ttcaacaatg gctttacgca tgacttcaaa
      541 tctctttggt acgacatgtt caattctttc tttaaatagt tcggaggttg ccacggtcaa
      601 ttgcataccc tgagtggaac tcacatcctt tttaatatcg ctgacaacta ggacacaagc
      661 tttcatctga ggccagtcag agctgtctgc gatttgtact gccatggaat catgaccatc
      721 ttcagctttt cccatttccc aggccacgta tccgccaaac aacgatctac aagctgaacc
      781 agaccccttt cttgctattc tagatatttc tgaagttgac tgtggtaatt ggtataactt
      841 agcaattgca gagaccaatg cagcaaagcc agcagcggag gaagctaaac cagctgctgt
      901 aggaaagtta ttttcggaga caatgtggag tttccattga gataatgtgg gcaatgaggc
      961 gtccttcgat tccatttcct ttcttaattg g
//
