LOCUS       sequence-167737-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter 
            for isopentenol production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   George KW, Thompson MG, Kang A, Baidoo E, Wang G, Chan LJ, Adams PD,
            Petzold CJ, Keasling JD, Lee TS
  TITLE     Metabolic engineering for the high-yield production of 
            isoprenoid-based C(5) alcohols in E. coli.
  JOURNAL   Sci Rep. 2015 Jun 8;5:11128. doi: 10.1038/srep11128.
  PUBMED    26052683
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-8"; volume: "5:11128. doi"; pages: " 
            10.1038/srep11128"
COMMENT     Sequence Label: JBEI-6823
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(61..66,67..84,85..91)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    99..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(850..861)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgtttagg caccccaggc
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      121 gaattcaaaa gatcttagga ggaatataaa atgaaaaatt gtgtcatcgt cagtgcggta
      181 cgtactgcta tcggtagttt taacggttca ctcgcttcca ccagcgccat cgacctgggg
      241 gcgacagtaa ttaaagccgc cattgaacgt gcaaaaatcg attcacaaca cgttgatgaa
      301 gtgattatgg gtaacgtgtt acaagccggg ctggggcaaa atccggcgcg tcaggcactg
      361 ttaaaaagcg ggctggcaga aacggtgtgc ggattcacgg tcaataaagt atgtggttcg
      421 ggtcttaaaa gtgtggcgct tgccgcccag gccattcagg caggtcaggc gcagagcatt
      481 gtggcggggg gtatggaaaa tatgagttta gccccctact tactcgatgc aaaagcacgc
      541 tctggttatc gtcttggaga cggacaggtt tatgacgtaa tcctgcgcga tggcctgatg
      601 tgcgccaccc atggttatca tatggggatt accgccgaaa acgtggctaa agagtacgga
      661 attacccgtg aaatgcagga tgaactggcg ctacattcac agcgtaaagc ggcagccgca
      721 attgagtccg gtgcttttac agccgaaatc gtcccggtaa atgttgtcac tcgaaagaaa
      781 accttcgtct tcagtcaaga cgagttcccg aaagcgaact caacggctga agcgttaggt
      841 gcattgcgcc cggccttcga taaagcagga acagtcaccg ctgggaacgc gtctggtatt
      901 aacgacggtg ctgccgctct ggtgattatg gaagaatctg cggcgct
//
