LOCUS       sequence-167803-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing HA epitope fused to C-terminus of YFP 
            without pA for imaging, epitope labeling/purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-YFP-HA no-pA
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..175)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(178..180,181..693)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(220..241)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(481..500)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     828..849
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctctagagc tcgaattcta caggtcacta ataccatcta agtagttggt tcatagtgac
       61 tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      121 atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa agtggcggtg
      181 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      241 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      301 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      361 accaccttcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      421 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      481 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      541 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      601 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      661 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      721 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      781 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      841 gagttcgtga ccgccgccgg
//
