LOCUS       sequence-167963-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.I35S
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     499..518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     752..769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(928..955)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1047..1133)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1182..1204
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1196..1213
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1197..1213
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1229..1328
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1331..3676
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGSHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(3678..3777)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3794..3813)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3795..3813)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3818..3834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3818..3834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4265..5056
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
       61 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      781 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      841 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      901 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      961 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     1021 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     1081 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
     1141 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
     1201 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
     1261 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
     1321 agttggcacc atggctactc aagctgattt gatggagttg gacatggcca tggaaccaga
     1381 cagaaaagcg gctgttagtc actggcagca acagtcttac ctggactctg gaagccattc
     1441 tggtgccact accacagctc cttctctgag tggtaaaggc aatcctgagg aagaggatgt
     1501 ggatacctcc caagtcctgt atgagtggga acagggattt tctcagtcct tcactcaaga
     1561 acaagtagct gatattgatg gacagtatgc aatgactcga gctcagaggg tacgagctgc
     1621 tatgttccct gagacattag atgagggcat gcagatccca tctacacagt ttgatgctgc
     1681 tcatcccact aatgtccagc gtttggctga accatcacag atgctgaaac atgcagttgt
     1741 aaacttgatt aactatcaag atgatgcaga acttgccaca cgtgcaatcc ctgaactgac
     1801 aaaactgcta aatgacgagg accaggtggt ggttaataag gctgcagtta tggtccatca
     1861 gctttctaaa aaggaagctt ccagacacgc tatcatgcgt tctcctcaga tggtgtctgc
     1921 tattgtacgt accatgcaga atacaaatga tgtagaaaca gctcgttgta ccgctgggac
     1981 cttgcataac ctttcccatc atcgtgaggg cttactggcc atctttaagt ctggaggcat
     2041 tcctgccctg gtgaaaatgc ttggttcacc agtggattct gtgttgtttt atgccattac
     2101 aactctccac aaccttttat tacatcaaga aggagctaaa atggcagtgc gtttagctgg
     2161 tgggctgcag aaaatggttg ccttgctcaa caaaacaaat gttaaattct tggctattac
     2221 gacagactgc cttcaaattt tagcttatgg caaccaagaa agcaagctca tcatactggc
     2281 tagtggtgga ccccaagctt tagtaaatat aatgaggacc tatacttacg aaaaactact
     2341 gtggaccaca agcagagtgc tgaaggtgct atctgtctgc tctagtaata agccggctat
     2401 tgtagaagct ggtggaatgc aagctttagg acttcacctg acagatccaa gtcaacgtct
     2461 tgttcagaac tgtctttgga ctctcaggaa tctttcagat gctgcaacta aacaggaagg
     2521 gatggaaggt ctccttggga ctcttgttca gcttctgggt tcagatgata taaatgtggt
     2581 cacctgtgca gctggaattc tttctaacct cacttgcaat aattataaga acaagatgat
     2641 ggtctgccaa gtgggtggta tagaggctct tgtgcgtact gtccttcggg ctggtgacag
     2701 ggaagacatc actgagcctg ccatctgtgc tcttcgtcat ctgaccagcc gacaccaaga
     2761 agcagagatg gcccagaatg cagttcgcct tcactatgga ctaccagttg tggttaagct
     2821 cttacaccca ccatcccact ggcctctgat aaaggctact gttggattga ttcgaaatct
     2881 tgccctttgt cccgcaaatc atgcaccttt gcgtgagcag ggtgccattc cacgactagt
     2941 tcagttgctt gttcgtgcac atcaggatac ccagcgccgt acgtccatgg gtgggacaca
     3001 gcagcaattt gtggaggggg tccgcatgga agaaatagtt gaaggttgta ccggagccct
     3061 tcacatccta gctcgggatg ttcacaaccg aattgttatc agaggactaa ataccattcc
     3121 attgtttgtg cagctgcttt attctcccat tgaaaacatc caaagagtag ctgcaggggt
     3181 cctctgtgaa cttgctcagg acaaggaagc tgcagaagct attgaagctg agggagccac
     3241 agctcctctg acagagttac ttcactctag gaatgaaggt gtggcgacat atgcagctgc
     3301 tgttttgttc cgaatgtctg aggacaagcc acaagattac aagaaacggc tttcagttga
     3361 gctgaccagc tctctcttca gaacagagcc aatggcttgg aatgagactg ctgatcttgg
     3421 acttgatatt ggtgcccagg gagaacccct tggatatcgc caggatgatc ctagctatcg
     3481 ttcttttcac tctggtggat atggccagga tgccttgggt atggacccca tgatggaaca
     3541 tgagatgggt ggccaccacc ctggtgctga ctatccagtt gatgggctgc cagatctggg
     3601 gcatgcccag gacctcatgg atgggctgcc tccaggtgac agcaatcagc tggcctggtt
     3661 tgatactgac ctgtaattgc caactttctt gtacaaagtt ggcattataa gaaagcattg
     3721 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     3781 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     3841 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     3901 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     3961 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     4021 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     4081 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     4141 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     4201 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     4261 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     4321 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     4381 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     4441 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     4501 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     4561 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     4621 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     4681 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     4741 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     4801 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     4861 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     4921 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     4981 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     5041 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     5101 gtcgttccac tgagcgtcag accccgtaga aaagatcaa
//
