LOCUS       sequence-168067-        5877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse neurofilament protein L cDNA mammalian expression construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Yan Y, Jensen K, Brown A
  TITLE     The polypeptide composition of moving and stationary neurofilaments 
            in cultured sympathetic neurons.
  JOURNAL   Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309.
  PUBMED    17285620
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Motil 
            Cytoskeleton."; date: "2007-04"; volume: "64(4)"; pages: "299-309"
COMMENT     Sequence Label: pmNFL
FEATURES             Location/Qualifiers
     source          1..5877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2665..2786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2702..2721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2756..2775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2793..3248)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2930..2951)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3142..3161
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3275..3379
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3381..3738
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3381..3572
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3404..3424)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3589..3724
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3773..4567
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3773..4567
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3827..3846)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4437..4456
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4755..4774)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4799..4846
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5175..5763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5664..5683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagtt ctctctaggt cccccatctc cgccccgggc ctgaggggcg
      661 caccggccgc caccatgagt tcgttcggct acgatccgta cttttcgacc tcctacaagc
      721 ggcgctatgt ggagacgccc cgggtgcaca tctccagcgt gcgcagcggc tacagcacgg
      781 cgcgctccgc gtactccagc tactccgcgc cggtctcctc ctcgctgtcc gtgcgccgca
      841 gctactcgtc cagctctggc tctttgatgc ccagcctgga gaatctcgat ctgagccagg
      901 tagccgccat cagcaacgac ctcaagtcta tccgcacaca agagaaggca cagctgcagg
      961 acctcaacga tcgcttcgcc agcttcatcg agcgcgtgca cgagctggag cagcagaaca
     1021 aggtcctgga agccgagctg ttggtgctgc gccagaaaca ctctgagcct tcccgcttcc
     1081 gcgccctgta cgagcaggag atccgcgatc tgcggctggc agcggaagac gccactaacg
     1141 agaagcaggc gctgcagggc gagcgcgagg ggctggagga gactctgcgc aacctgcagg
     1201 ctcgctatga ggaagaagtg ctgagccgcg aggacgccga gggccggctg atggaagcgc
     1261 gcaaaggtgc ggatgaggcc gcgctcgccc gcgccgagct ggagaagcgc atcgacagcc
     1321 tgatggacga gatagctttc ctgaagaagg tgcacgagga agagatcgcc gagctgcagg
     1381 ctcagatcca gtatgctcag atctccgtgg agatggacgt gtcctccaag cccgacctct
     1441 ccgccgctct caaggacatc cgcgctcagt acgagaagct ggccgccaag aacatgcaga
     1501 acgccgaaga gtggttcaag agccgcttca ccgtgctaac cgagagcgcc gccaagaaca
     1561 ccgacgctgt gcgcgctgcc aaggacgagg tgtcggaaag ccgccgcctg ctcaaggcta
     1621 agaccctgga gatcgaagcc tgccggggta tgaacgaagc tctggagaag cagctgcagg
     1681 agctagagga caagcagaat gcagacatta gcgccatgca ggacacaatc aacaaactgg
     1741 agaatgagct gagaagcacg aagagcgaga tggccaggta cctgaaggag taccaggacc
     1801 tcctcaatgt caagatggcc ttggacatcg agattgcagc ttacagaaaa ctcttggaag
     1861 gcgaagagac caggctcagt ttcaccagcg tgggtagcat aaccagcggc tactctcaga
     1921 gctcgcaggt cttcggccgt tctgcttaca gtggcttgca gagcagctcc tacttgatgt
     1981 ctgctcgctc tttcccagcc tactatacca gccacgtcca ggaagagcag acagaggtcg
     2041 aggagaccat tgaggctacg aaagctgagg aggccaagga tgagcccccc tctgaaggag
     2101 aagcagaaga ggaggagaag gagaaagagg agggagagga agaggaaggc gctgaggagg
     2161 aagaagctgc caaggatgag tctgaagaca caaaagaaga agaagaaggt ggtgagggtg
     2221 aggaggaaga caccaaagaa tctgaagagg aagagaagaa agaggagagt gctggagagg
     2281 agcaggtggc taagaagaaa gattgagccc tattcccaac tattccagga aaagttctcc
     2341 ccaatcaggt caacctcatc accaaccaac cagttgagtt ccagatccta tacaaattaa
     2401 gaagtcaata catgtataat tctgagatga cttaggttgg actttcaatg ttgtgctatg
     2461 aatttcctcc ttacgcagag tatctgtttg cttgcagagt ggctgaattc tgcagtcgac
     2521 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     2581 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2641 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2701 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2761 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     2821 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     2881 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     2941 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3001 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3061 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3121 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3181 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3241 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3301 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3361 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3421 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3481 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3541 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3601 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3661 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3721 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     3781 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3841 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3901 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     3961 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4021 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4081 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4141 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4201 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4261 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4321 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4381 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4441 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4501 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4561 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4621 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4681 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     4741 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4801 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     4861 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     4921 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     4981 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5041 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5101 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5161 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5221 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5281 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     5341 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5401 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5461 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5521 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5581 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5641 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5701 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5761 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5821 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
