LOCUS       sequence-168079-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive CRISPR interference (CRISPRi) knock in construct into 
            the AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-NC-CRISPRi (Gen3)
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..115)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(122..142)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(155..181)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(161..187)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattcacg cgttaggatc
       61 cgctgctgcc gttgctccca ctaccaatgc cgtccacttt ccgctttttc ttaggatctt
      121 ccaccttgcg ttttttcttg ggggagccgc tgcccaggct ggcataatcg ggcacgtcat
      181 agggataggc gtcgcctccc agctgagaca ggtcgatccg tgtctcgtac aggccggtga
      241 tgctctggtg gatcagggtg gcgtccagca cctctttggt gctggtgtac ctcttccggt
      301 cgatggtggt gtcaaagtac ttgaaggcgg caggggctcc cagattggtc agggtaaaca
      361 ggtggatgat attctcggcc tgctctctga taggcttgtc tctgtgcttg ttgtaggcgc
      421 tcagcacctt gtccagatta gcgtcggcca ggatcactct cttggagaac tcgctgatct
      481 gctcgatgat ctcgtccagg tagtgtttgt gctgttccac aaacagctgt ttctgctcat
      541 tatcctcggg ggagcccttc agcttctcat agtggctggc caggtacagg aagttcacat
      601 atttggaggg cagggccagt tcgtttccct tctgcagttc gccggcagag gccagcattc
      661 tcttccggcc gttttccagc tcgaacaggg agtacttagg cagcttgatg atcaggtcct
      721 ttttcacttc tttgtagccc ttggcttcca gaaagtcgat gggattcttc tcgaagctgc
      781 ttctttccat gatggtgatc cccagcagct ctttcacact cttcagtttc ttggacttgc
      841 ccttttccac tttggccacc accagcacag aataggccac ggtggggctg tcgaagccgc
      901 cgtacttctt agggtcccag tccttctttc tggcgatcag cttgtcgctg ttcctcttgg
      961 gcag
//
