LOCUS       sequence-168083-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EE-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
  TITLE     Increasing the genome-targeting scope and precision of base editing 
            with engineered Cas9-cytidine deaminase fusions.
  JOURNAL   Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
  PUBMED    28191901
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803."
COMMENT     Sequence Label: pBK-EE-BE3
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..45)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(58..306)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 ggtgatggtg atgatgaccg gttagacttt cctcttcttc ttgggagaac caccagagag
       61 catcttaatc ttgttctcac cgttgctatc ctgtatgacc agagcccaag gcttgtattc
      121 aggggcgtcg ctagtcagaa gcatgacatt ctcgtcggtg ctctcgtcgt aggcggtgtg
      181 cacgagtata tcgctttccg gcttgttccc aatgacttct tccacctcct ctgggagcat
      241 gaggatggat tcctggataa ccagttgctt accggtctcc ttttcaataa tatctgacag
      301 attagtagaa ccaccagagt cacccccaag ctgtgacaaa tctatccgag tttcatataa
      361 tcccgtgatg gattggtgaa tcagtgtcgc gtctagcacc tccttggtag aagtgtatcg
      421 tttgcgatct atcgttgtgt caaaatactt gaatgcggct ggagcgccga ggttggtaag
      481 agtaaacaaa tggataatat tttccgcctg ctcacgtatg ggtttatccc tgtgcttgtt
      541 gtatgcgctt aatactttgt ccagattggc atcagctagg atgactctct tactgaattc
      601 cgaaatttgc tctatgattt cgtcgagata atgtttgtgc tgctcaacaa aaagttgctt
      661 ctgttcgtta tcttcaggtg aacctttcaa cttctcgtaa tgggacgcta aatacaggaa
      721 attcacgtat ttagacggta gtgcgagttc gttccccttt tgaagctctc cggcgctagc
      781 caacatccgt tttcggccat tttctaactc aaacagacta tactttggta gtttaattat
      841 gagatccttt tttacttcct tgtaaccttt cgcctcaagg aagtcgatgg ggttcttttc
      901 aa
//
