LOCUS       sequence-168107-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-inducible CRISPR interference (CRISPRi) knock in construct into 
            the AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-NDi-CRISPRi (Gen2)
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgcctctct cggccttggt cagattgtcg aacttcctct gggtaatcag cttggcattc
       61 agcagctggc gccagtagtt cttcatcttc ttcacgacct cttcggaggg cacgttgtcg
      121 ctcttgcccc ggttcttgtc gctccgagtc agcactttgt tatcgatgga gtcgtccttc
      181 agaaagctct gaggcacgat agcgtccaca tcgtagtcgg acagccggtt gatgtccagt
      241 tcctggtcca cgtacatatc ccgcccattc tgcaggtagt acaggtacag cttctcgttc
      301 tgcagctggg tgttttccac ggggtgttct ttcaggatct ggctgcccag ctctttgatg
      361 ccctcttcga tccgcttcat tctctcgcgg ctgttcttct gtcccttctg ggtggtctgg
      421 ttctctctgg ccatttcgat cacgatgttc tcgggcttgt gccggcccat cactttcacg
      481 agctcgtcca ccaccttcac tgtctgcagg atgcccttct taatggcggg gctgccggcc
      541 agattggcaa tgtgctcgtg caggctatcg ccctggccgg acacctgggc tttctggatg
      601 tcctctttaa aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct gttggcgaag
      661 ccgtcggact tcaggaaatc caggattgtc ttgccggact gcttgtcccg gatgccgttg
      721 atcagcttcc ggctcagcct gccccagccg gtgtatctcc gccgcttcag ctgcttcatc
      781 actttgtcgt cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat ctctctgtcc
      841 tcaaacagtg tcagggtcag cacgatatct tccagaatgt cctcgttttc ctcattgtcc
      901 aggaagtcct tgtccttgat aattttcagc agatcgtggt atgtgc
//
