LOCUS       sequence-168110-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Chen ZJ, Ai HW
  TITLE     A highly responsive and selective fluorescent probe for imaging 
            physiological hydrogen sulfide.
  JOURNAL   Biochemistry. 2014 Sep 23;53(37):5966-74. doi: 10.1021/bi500830d. 
            Epub 2014 Sep 5.
  PUBMED    25141269
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi500830d";
            journalName: "Biochemistry"; date: "2014-09-23- 23"; volume: "53"; 
            issue: "37"; pages: "5966-74"
COMMENT     Sequence Label: pBAD-hsGFP
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     305..326
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(438..459)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             831..848
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttg ggctaacagg
       61 aggaattaac catgggctcg agcccgtaca acagccacaa ggtctatatc accgccgaca
      121 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacgtggag gacggcagcg
      181 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      241 ccgacaacca ctacctgagc acccagtccg tgctgagcaa agaccccaac gagaagcgcg
      301 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
      361 tgtacaaggt ggacggcgga tccggtggca ccggagtgag caagggcgag gagctgttca
      421 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      481 tgaggggcga aggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca
      541 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc tagggcgtgc
      601 agtgcttctc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      661 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcacc tacaagaccc
      721 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      781 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactgg caccatcacc
      841 atcaccatta agcttggctg ttt
//
