LOCUS       sequence-16823-e        1314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1314)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD177.62
FEATURES             Location/Qualifiers
     source          1..1314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             482..502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(576..595)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gntgngcctg cnggtcgact ctagaggatc cccggggtac ctaatttgat cgataatatg
       61 tacatattnc gttattcgtt ccggtatcgt acctccacct accataaatc ccatacccta
      121 catctttatc gattttttgc aaattacgag cgttgtaggg ggcggagcga taggtcctat
      181 aggttttggt atatcatcat tcattcattc attggtacat tcatttaccc accttcctct
      241 ttctgagctt ctctggagtt ctgtgcttcc tttttccctt atctttatac tgtaattttt
      301 aactttcagg cattgattgg atccccggga ttggccaaag gacccaaagg tatgtttcga
      361 atgatactaa cataacatag aacattttca ggaggaccct tggctagagg atccccggga
      421 ttggccaaag gatccaaagg aggacccttg gagggtaccg agctcagaaa aaatgactgc
      481 tccaaagaag aagcgtaagg taccggctag attagataaa agtaaagtga ttaacagcgc
      541 attagagcta cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
      601 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
      661 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
      721 aagctggcaa gattttttac gtaagtttaa acatattgtt actaactaac cttatacatt
      781 taaattttca ggtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
      841 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
      901 attagccttt ttatgccaac naggtttttc actagagaat gcattatatg cactcagcgc
      961 tgtggggcat ttttacttta ggttgcgtat tggaagatca agagcatcna gtcgctaaag
     1021 anaaagggna acncctacta ctgaaagnag ccgccattat tacgacagct atcgaattat
     1081 tngatcncag tangttnaac gngggnanac taactaccgg cananttaaa ttttcaaagg
     1141 ggcaannccg ccctcntann cgncctgaat ggacaatggg gataaaaaac acttaanggn
     1201 angggggncc caaaaaggaa aaaantttcc ggnatgnccc antctggtga ataaaggggg
     1261 ggtaagggnc aaatttcggc cngggaaggg ganggnggcc ntcggaannn ncct
//
