LOCUS       sequence-168388-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducibile Tra* Promoter driving sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs074-Ptra*
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..84
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     146..163
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(193..906)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             913..921
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaattattt tttataatgc
       61 caactttgta caagaaagct gggtcgaatt gattaccgcc tttgagtgag ctgataccgc
      121 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct gaggagcgag
      181 ttaggatcct catttgtaca gttcatccat accatgcgtg atgcccgctg cggttacgaa
      241 ctccagcaga accatatgat cgcgtttctc gttcggatct ttagacagaa cgctttgcgt
      301 gctcagatag tgattgtctg gcagcagaac aggaccatca ccgattggag tgttttgctg
      361 gtagtgatca gccagctgca cgctgccatc ctccacgttg tggcgaattt taaaattcgc
      421 tttaatgcca tttttttgtt tatcggcggt gatgtaaaca ttgtggctgt taaaattgta
      481 ttccagctta tggcccagga tattgccgtc ttctttaaag tcaatgcctt tcagctcaat
      541 gcggtttacc agggtatcgc cttcaaattt cacttccgca cgcgttttgt acgtgccgtc
      601 atccttaaag gaaatcgtgc gttcctgcac atagccttcc ggcatggcgg acttgaagaa
      661 gtcatgctgc ttcatatggt ccggataacg agcaaagcac tgaacaccat aagtcagcgt
      721 cgttaccaga gtcggccaag gtaccggcag tttaccagta gtacagatga acttcagcgt
      781 cagtttacca ttagttgcgt caccttcacc ctcgccacgc acggaaaact tatgaccgtt
      841 gacatcacca tccagttcca ccagaatagg gacgacacca gtgaacagct cttcgccttt
      901 acgcatattg tcacctcctt atattcgact ataacaaacc at
//
