LOCUS       sequence-168495-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Drosophila p53 KRKR mutant in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky 
            MH, Collavin L
  TITLE     Modification of Drosophila p53 by SUMO modulates its transactivation
            and pro-apoptotic functions.
  JOURNAL   J Biol Chem. 2008 Jul 25. 283(30):20848-56.
  PUBMED    18492669
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jul 25. 283(30):20848-56."
COMMENT     Sequence Label: pAc-HT-Dmp53KRKR
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..48
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(71..88)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..139)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cgatcgaggc tgatcagcga gctcaggcct tagaaggcac agtcgaggct gatcagcggg
       61 tttaaactca atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag
      121 ggttagggat aggcttacct tcgaagggcc ctctagaggc gcgccttatc atggcagctc
      181 gtaggcacgt ttcttaaggc tcagcaattt gttggcatgg cgacgtagat tctcttggtt
      241 gggattgcgc aggacttcag ccgccgcctc cttaatcatg ccctcgatgc tctgcagcag
      301 ccattcctta ttggggcacg taatagccag acggtaatcg ccatccggtg tccgcgacac
      361 gttccactct gcggcggagt cgtcgcagtc tcggctatca ttgctctccg tgtcctccgt
      421 ccttatagca atgcaccgac gcaccttgga cggctcatct tcttcagcgg cttccggcac
      481 ggacttgcgc ttcttgctat tgagctggcg ttcgtcttgg atgcgatccc gcttggggca
      541 cgtacatatt ttaacatgta taacatgctg tcccacgata tcgccgcatg ctttctccag
      601 gcagaagact aaggaagttt cttttcgccc gatacacgag ttttggcaga cgaacttgaa
      661 ggccagggtc tggcgcgtga gcccactgcg ggttacagac cggctcatgt tcagggggac
      721 tacaacggaa aaacgctcgg aaattccctt gccctgagca tttccacaat atacactgtt
      781 gggattctcg ctgcgcagca agctctcgcg cattt
//
