LOCUS       sequence-168580-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses p63 (580 a.a.) tagged with mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   van Unen J, Yin T, Wu YI, Mastop M, Gadella TW Jr, Goedhart J
  TITLE     Kinetics of recruitment and allosteric activation of ARHGEF25 
            isoforms by the heterotrimeric G-protein Galphaq.
  JOURNAL   Sci Rep. 2016 Nov 11;6:36825. doi: 10.1038/srep36825.
  PUBMED    27833100
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-11-11"; volume: "6:36825. doi"; pages: " 
            10.1038/srep36825"
COMMENT     Sequence Label: p63RhoGEF580-mVenus-N1
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..169
                     /codon_start=1
                     /product="palmitoylation signal consisting of the first 29 
                     amino acids of p63RhoGEF (Goedhart et al., 2013)"
                     /label=palmitoylation signal
                     /translation="MRGGHKGGRCACPRVIRKVLAKCGCCFAR"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa
       61 ttctgcagtc gacggtacca ccatgcgggg ggggcacaaa gggggtcgct gtgcctgtcc
      121 ccgtgtgatc cgaaaagtgc tggcaaaatg cggctgctgc ttcgcccggg ggggacgtga
      181 atcctattcc attgcgggca gtgaggggag tatatcggct tctgctgcct ccggtctggc
      241 tgccccctct ggccccagct ctggcctcag ctctggcccc tgttccccag gccccccagg
      301 gcccgtcagt ggcctgagga gatggttgga tcattccaaa cattgtctca gtgtggaaac
      361 tgaggcagac agtggtcagg caggaccata tgagaactgg atgttggagc cagctctagc
      421 cacaggagag gagctgccgg aactgacctt gctgaccaca ctgttggagg gccctggaga
      481 taagacgcag ccacctgaag aggagacttt gtcccaagcc cctgagagtg aggaggaaca
      541 gaagaagaag gctctggaaa ggagtatgta tgtcctgagt gaactggtag aaacagagaa
      601 aatgtacgtg gacgacttgg ggcagattgt ggagggttat atggccacca tggctgctca
      661 gggggtcccc gagagtcttc
//
