LOCUS       sequence-168582-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mVenus tagged with a membrane localization sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   van Unen J, Botman D, Yin T, Wu YI, Hink MA, Gadella TWJ Jr, Postma 
            M, Goedhart J
  TITLE     The C-terminus of the oncoprotein TGAT is necessary for plasma 
            membrane association and efficient RhoA-mediated signaling.
  JOURNAL   BMC Cell Biol. 2018 Jun 7;19(1):6. doi: 10.1186/s12860-018-0155-2.
  PUBMED    29879899
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2018-06-7"; volume: "19(1):6. doi"; pages: " 
            10.1186/s12860-018-0155-2"
COMMENT     Sequence Label: pLck-mVenus-C1
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(84..86,87..89,90..800)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(129..150)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(390..409)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     737..758
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagccaccat gggctgcgtg tgcagcagca
       61 accccgagct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
      121 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      181 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
      241 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg
      301 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      361 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      421 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      481 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      541 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
      601 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      661 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca
      721 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      781 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg aa
//
