LOCUS       sequence-168708-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV/TO 
            promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV/TO Puro DEST (670-1)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..204
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(229..234,235..252,253..259)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(379..398)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgactag tccagtgtgg
       61 tggaattctg cagatatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
      121 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      181 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
      241 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
      301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgt
//
