LOCUS       sequence-168746-        5724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SYCP1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SYCP1_p.E773K
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3603..3702)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3719..3738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3720..3738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3743..3759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3743..3759)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4190..4981
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5074..5662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5563..5582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggaaaagc aaaagccctt tgcattgttc gtaccaccga gatcaagcag
      721 cagtcaggtg tctgcggtga aacctcagac cctgggaggc gattccactt tcttcaagag
      781 tttcaacaaa tgtactgaag atgattttga gtttccattt gcaaagacta atctctccaa
      841 aaatggggaa aacattgatt cagatcctgc tttacaaaaa gttaatttct tgcccgtgct
      901 tgagcaggtt ggtaattctg actgtcacta tcaggaagga ctaaaagact ctgatttgga
      961 gaattcagag ggattgagca gagtgtattc aaaactgtat aaggaggctg aaaagataaa
     1021 aaaatggaaa gtaagtacag aagctgaact gagacagaaa gaaagtaagt tgcaagaaaa
     1081 cagaaagata attgaagcac agcgaaaagc cattcaggaa ctgcaatttg gaaatgaaaa
     1141 agtaagtttg aaattagaag aaggaataca agaaaataaa gatttaataa aagagaataa
     1201 tgccacaagg catttatgta atctactcaa agaaacctgt gctagatctg cagaaaagac
     1261 aaagaaatat gaatatgaac gggaagaaac caggcaagtt tatatggatc taaataataa
     1321 cattgagaaa atgataacag cttttgagga acttcgtgtg caagctgaga attccagact
     1381 ggaaatgcat tttaagttaa aggaagatta tgaaaaaatc caacaccttg aacaagaata
     1441 caagaaggaa ataaatgaca aggaaaagca ggtatcacta ctattgatcc aaatcactga
     1501 gaaagaaaat aaaatgaaag atttaacatt tctgctagag gaatccagag ataaagttaa
     1561 tcaattagag gaaaagacaa aattacagag tgaaaactta aaacaatcaa ttgagaaaca
     1621 gcatcatttg actaaagaac tagaagatat taaagtgtca ttacaaagaa gtgtgagtac
     1681 tcaaaaggct ttagaggaag atttacagat agcaacaaaa acaatttgtc agctaactga
     1741 agaaaaagaa actcaaatgg aagaatctaa taaagctaga gctgctcatt cgtttgtggt
     1801 tactgaattt gaaactactg tctgcagctt ggaagaatta ttgagaacag aacagcaaag
     1861 attggaaaaa aatgaagatc aattgaaaat acttaccatg gagcttcaaa agaaatcaag
     1921 tgagctggaa gagatgacta agcttacaaa taacaaagaa gtagaacttg aagaattgaa
     1981 aaaagtcttg ggagaaaagg aaacactttt atatgaaaat aaacaatttg agaagattgc
     2041 tgaagaatta aaaggaacag aacaagaact aattggtctt ctccaagcca gagagaaaga
     2101 agtacatgat ttggaaatac agttaactgc cattaccaca agtgaacagt attattcaaa
     2161 agaggttaaa gatctaaaaa ctgagcttga aaacgagaag cttaagaata ctgaattaac
     2221 ttcacactgc aacaagcttt cactagaaaa caaagagctc acacaggaaa caagtgatat
     2281 gaccctagaa ctcaagaatc agcaagaaga tattaataat aacaaaaagc aagaagaaag
     2341 gatgttgaaa caaatagaaa atcttcaaga aacagaaacc caattaagaa atgaactaga
     2401 atatgtgaga gaagagctaa aacagaaaag agatgaagtt aaatgtaaat tggacaagag
     2461 tgaagaaaat tgtaacaatt taaggaaaca agttgaaaat aaaaacaagt atattgaaga
     2521 acttcagcag gagaataagg ccttgaaaaa aaaaggtaca gcagaaagca agcaactgaa
     2581 tgtttatgag ataaaggtca ataaattaga gttagaacta gaaagtgcca aacagaaatt
     2641 tggagaaatc acagacacct atcagaaaga aattgaggac aaaaagatat cagaagaaaa
     2701 tcttttggaa gaggttgaga aagcaaaagt aatagctgat gaagcagtaa aattacagaa
     2761 agaaattgat aagcgatgtc aacataaaat agctgaaatg gtagcactta tggaaaaaca
     2821 taagcaccaa tatgataaga tcattgaaga aagagactca gaattaggac tttataagag
     2881 caaagaacaa gaacagtcat cactgagagc atctttggag attgaactat ccaatctcaa
     2941 agctgaactt ttgtctgtta agaagcaact tgaaatagaa agagaaaaga aggaaaaact
     3001 caaaagagag gcaaaagaaa acacagctac tcttaaagaa aaaaaagaca agaaaacaca
     3061 aacattttta ttggaaacac ctgaaattta ttggaaattg gattctaaag cagttccttc
     3121 acaaactgta tctcgaaatt tcacatcagt tgatcatggc atatccaaag ataaaagaga
     3181 ctatctgtgg acatctgcca aaaatacttt atctacacca ttgccaaagg catatacagt
     3241 gaagacacca acaaaaccaa aactacagca aagagaaaac ttgaatatac ccattgaaga
     3301 aagtaaaaaa aagagaaaaa tggcctttga atttgatatt aattcagata gttcagaaac
     3361 tactgatctt ttgagcatgg tttcagaaga agagacattg aaaacactgt ataggaacaa
     3421 taatccacca gcttctcatc tttgtgtcaa aacaccaaaa aaggcccctt catctctaac
     3481 aacccctgga tctacactga agtttggagc tataagaaaa atgcgggagg accgttgggc
     3541 tgtaattgct aaaatggata gaaaaaaaaa actaaaagaa gctgaaaagt tatttgtttg
     3601 attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     3661 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     3721 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     3781 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     3841 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     3901 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     3961 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     4021 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     4081 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     4141 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     4201 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     4261 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     4321 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     4381 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     4441 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     4501 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     4561 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     4621 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     4681 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     4741 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     4801 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     4861 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     4921 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     4981 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     5041 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5101 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5161 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5221 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5281 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5341 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5401 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5461 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5521 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5581 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5641 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5701 ttgctggcct tttgctcaca tgtt
//
