LOCUS       sequence-168990-        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed N-term tagged APEX2 on Sec61B in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW
  TITLE     APEX Fingerprinting Reveals the Subcellular Localization of Proteins
            of Interest.
  JOURNAL   Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064.
  PUBMED    27184847
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064."
COMMENT     Sequence Label: pCDNA3_V5-APEX2-Sec61B
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..95
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        76..94
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..164
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             165..911
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 ccccgggggt ggaggtcaaa taagcagagc tctctggcta actagagaac ccactgctta
       61 ctggcttatc gaaattaata cgactcacta tagggagacc caagcttgcg gccgccacca
      121 tgggcaagcc catccccaac cccctgctgg gcctggacag caccggaaag tcttacccaa
      181 ctgtgagtgc tgattaccag gacgccgttg agaaggcgaa gaagaagctc agaggcttca
      241 tcgctgagaa gagatgcgct cctctaatgc tccgtttggc attccactct gctggaacct
      301 ttgacaaggg cacgaagacc ggtggaccct tcggaaccat caagcaccct gccgaactgg
      361 ctcacagcgc taacaacggt cttgacatcg ctgttaggct tttggagcca ctcaaggcgg
      421 agttccctat tttgagctac gccgatttct accagttggc tggcgttgtt gccgttgagg
      481 tcacgggtgg acctaaggtt ccattccacc ctggaagaga ggacaagcct gagccaccac
      541 cagagggtcg cttgcccgat cccactaagg gttctgacca tttgagagat gtgtttggca
      601 aagctatggg gcttactgac caagatatcg ttgctctatc tgggggtcac actattggag
      661 ctgcacacaa ggagcgttct ggatttgagg gtccctggac ctctaatcct cttattttcg
      721 acaactcata cttcacggag ttgttgagtg gtgagaagga aggtctcctt cagctacctt
      781 ctgacaaggc tcttttgtct gaccctgtat tccgccctct cgttgataaa tatgcagcgg
      841 acgaagatgc cttctttgct gattacgctg aggctcacca aaagctttcc gagcttgggt
      901 ttgctgatgc caagctagca atgcctggtc cgacccccag tggcactaac gtgggatcct
      961 cagggcgctc tcccagcaaa gcagtggccg cccggggcgg cgggatccac tgtccggcag
     1021 aggaaaaatg ccagctgtgg ggacaaggag tgcaggccgc acaacctcgg caggaacccg
     1081 gggggggatg tgggcgattc tacacagaag attcactggg gctcaaagtt ggcccggttc
     1141 cagtaatggg a
//
