LOCUS       sequence-168992-        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed C-term tagged APEX2 on HMOX1 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW
  TITLE     APEX Fingerprinting Reveals the Subcellular Localization of Proteins
            of Interest.
  JOURNAL   Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064.
  PUBMED    27184847
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2016 May 11. pii: S2211-1247(16)30508-3. doi: 
            10.1016/j.celrep.2016.04.064."
COMMENT     Sequence Label: pCDNA3_HMOX1-V5-APEX2
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        15..33
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     15..32
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(67..813)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 gccgggaccc tagcatttag gtgacactat agaatagggc cctctagatg catgctcgag
       61 tcattaggca tcagcaaacc caagctcgga aagcttttgg tgagcctcag cgtaatcagc
      121 aaagaaggca tcttcgtccg ctgcatattt atcaacgaga gggcggaata cagggtcaga
      181 caaaagagcc ttgtcagaag gtagctgaag gagaccttcc ttctcaccac tcaacaactc
      241 cgtgaagtat gagttgtcga aaataagagg attagaggtc cagggaccct caaatccaga
      301 acgctccttg tgtgcagctc caatagtgtg acccccagat agagcaacga tatcttggtc
      361 agtaagcccc atagctttgc caaacacatc tctcaaatgg tcagaaccct tagtgggatc
      421 gggcaagcga ccctctggtg gtggctcagg cttgtcctct cttccagggt ggaatggaac
      481 cttaggtcca cccgtgacct caacggcaac aacgccagcc aactggtaga aatcggcgta
      541 gctcaaaata gggaactccg ccttgagtgg ctccaaaagc ctaacagcga tgtcaagacc
      601 gttgttagcg ctgtgagcca gttcggcagg gtgcttgatg gttccgaagg gtccaccggt
      661 cttcgtgccc ttgtcaaagg ttccagcaga gtggaatgcc aaacggagca ttagaggagc
      721 gcatctcttc tcagcgatga agcctctgag cttcttcttc gccttctcaa cggcgtcctg
      781 gtaatcagca ctcacagttg ggtaagactt tccggtgctg tccaggccca gcagggggtt
      841 gggggatggg cttgcctgct agcttcatgg cataaagccc tacagcaact gtcgccacca
      901 gaaagctgag tgtaaggacc catcggagaa gcggagcctg ggagcgggtg ttgagtgggg
      961 gcttccctct gggagtctcc acgggggcag aatcttgcac tttgttgctg gcccgctggc
     1021 gaagccctgg tgcccgtgag gggctctggt ccttggtgtc atgggtcagc agctcctgca
     1081 actcctcaaa gagctggatg ttgagcagga acgcagtctt ggcctcttct atcaccctct
     1141 gcctgactgc gggagtcatc tccagggagt tcatgcggga acggtaaaac tgcttgaact
     1201 tggtgg
//
