LOCUS       sequence-169003-        4008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HERPUD2 ,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HERPUD2_WT
FEATURES             Location/Qualifiers
     source          1..4008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1408..1507)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1524..1543)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1525..1543)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1548..1564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1548..1564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1995..2786
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2879..3467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3368..3387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3621..3638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3797..3824)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3916..4002)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggaccaaagt gggatggaga ttcctgtgac cctcatcatt aaagcaccga
      241 atcagaaata cagtgaccag actattagct gcttcttgaa ctggaccgtg gggaaactaa
      301 aaacgcatct atctaacgtt taccctagca aaccattgac gaaggatcag agattggtgt
      361 attcgggcag actgcttccc gatcatctgc agctgaaaga cattctcaga aaacaagatg
      421 agtatcatat ggttcatcta gtatgtactt ctcggactcc tcccagttct ccaaaatcca
      481 gcaccaatag agaaagtcat gaagcattga catccagcag caattctagt tcagatcatt
      541 caggatcaac aactccatca tctggtcaag aaaccttgtc tttagctgtg ggttcttcct
      601 cagaaggatt gaggcagcgt acccttccac aagcacaaac tgaccaagca cagagtcacc
      661 agtttccata tgtaatgcaa ggaaatgtag acaaccaatt tcctgggcaa gctgctccac
      721 ctggattccc agtgtatccc gcgtttagcc cactgcagat gctatggtgg caacagatgt
      781 atgctcatca gtattatatg cagtatcaag ctgcagtttc agctcaggcc acatcaaatg
      841 tcaacccaac ccagcctact acttcacagc ctctaaattt ggcacatgtt cctggagaag
      901 aacccccacc agctccaaac ctagtggccc aagaaaatcg acccatgaat gagaatgttc
      961 aaatgaatgc acagggaggt ccagtactaa atgaagaaga cttcaatcga gactggctag
     1021 actggatgta cacgttctca cgagctgcga ttctccttag cattgtatac ttctattctt
     1081 cttttagtcg gtttatcatg gtaatgggag ccatgctact ggtttattta caccaagctg
     1141 gatggtttcc ttttaggcaa gaaggaggtc atcagcaggc tcccaacaat aatgccgaag
     1201 ttaacaatga tgggcaaaat gcaaacaact tggaacttga agaaatggag cgtcttatgg
     1261 atgatgggct tgaagatgag agtggagaag atggaggtga agatgccagt gcaattcaaa
     1321 ggcctggatt aatggcttca gcttggtctt tcatcaccac cttctttact tcactaatac
     1381 cagaggggcc tccccaggtt gccaattgcc caactttctt gtacaaagtt ggcattataa
     1441 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1501 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     1561 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1621 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1681 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     1741 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     1801 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1861 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1921 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1981 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2041 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2101 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2161 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2221 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2281 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2341 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2401 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2461 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2521 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2581 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2641 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2701 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     2761 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     2821 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3661 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3721 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3781 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     3841 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     3901 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3961 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcct
//
