LOCUS       sequence-169047-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing mOrange2 fluorescent protein driven by 
            SFFV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Mohme M, Maire CL, Riecken K, Zapf S, Aranyossy T, Westphal M, 
            Lamszus K, Fehse B
  TITLE     Optical Barcoding for Single-Clone Tracking to Study Tumor 
            Heterogeneity.
  JOURNAL   Mol Ther. 2017 Jan 18. pii: S1525-0016(16)45496-1. doi: 
            10.1016/j.ymthe.2016.12.014.
  PUBMED    28109958
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2017 Jan 18. pii: S1525-0016(16)45496-1. doi: 
            10.1016/j.ymthe.2016.12.014."
COMMENT     Sequence Label: LeGO-mOrange2
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..765
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     protein_bind    complement(785..818)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(927..947)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cggatcccgc caccatggtg
       61 agcaagggcg aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcgc
      121 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      181 tacgagggct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      241 tgggacatcc tgtcccctca tttcacctac ggctccaagg cctacgtgaa gcaccccgcc
      301 gacatccccg actacttcaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      361 aactacgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      421 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtgatgcag
      481 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggtgccctg
      541 aagggcaaga tcaagatgag gctgaagctg aaggacggcg gccactacac ctccgaggtc
      601 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc
      661 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
      721 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagaatt cgtcgaggga
      781 cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc
      841 cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
      901 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
      961 ttaatgcctt tgtatcatgc tattgcttcc cgt
//
