LOCUS       sequence-169137-        4284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SLC2A3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SLC2A3_WT
FEATURES             Location/Qualifiers
     source          1..4284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2163..2262)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2279..2298)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2280..2298)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2303..2319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2303..2319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2750..3541
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3634..4222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4123..4142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggggacac agaaggtcac cccagctctg atatttgcca tcacagttgc
      721 tacaatcggc tctttccaat ttggctacaa cactggggtc atcaatgctc ctgagaagat
      781 cataaaggaa tttatcaata aaactttgac ggacaaggga aatgccccac cctctgaggt
      841 gctgctcacg tctctctggt ccttgtctgt ggccatattt tccgtcgggg gtatgatcgg
      901 ctccttttcc gtcggactct tcgtcaaccg ctttggcagg cgcaattcaa tgctgattgt
      961 caacctgttg gctgtcactg gtggctgctt tatgggactg tgtaaagtag ctaagtcggt
     1021 tgaaatgctg atcctgggtc gcttggttat tggcctcttc tgcggactct gcacaggttt
     1081 tgtgcccatg tacattggag agatctcgcc tactgccctg cggggtgcct ttggcactct
     1141 caaccagctg ggcatcgttg ttggaattct ggtggcccag atctttggtc tggaattcat
     1201 ccttgggtct gaagagctat ggccgctgct actgggtttt accatccttc ctgctatcct
     1261 acaaagtgca gcccttccat tttgccctga aagtcccaga tttttgctca ttaacagaaa
     1321 agaagaggag aatgctaagc agatcctcca gcggttgtgg ggcacccagg atgtatccca
     1381 agacatccag gagatgaaag atgagagtgc aaggatgtca caagaaaagc aagtcaccgt
     1441 gctagagctc tttagagtgt ccagctaccg acagcccatc atcatttcca ttgtgctcca
     1501 gctctctcag cagctctctg ggatcaatgc tgtgttctat tactcaacag gaatcttcaa
     1561 ggatgcaggt gttcaagagc ccatctatgc caccatcggc gcgggtgtgg ttaatactat
     1621 cttcactgta gtttctctat ttctggtgga aagggcagga agaaggactc tgcatatgat
     1681 aggccttgga gggatggctt tttgttccac gctcatgact gtttctttgt tattaaagga
     1741 taactataat gggatgagct ttgtctgtat tggggctatc ttggtctttg tagccttctt
     1801 tgaaattgga ccaggcccca ttccctggtt tattgtggcc gaactcttca gccagggccc
     1861 ccgcccagct gcgatggcag tggccggctg ctccaactgg acctccaact tcctagtcgg
     1921 attgctcttc ccctccgctg ctcactattt aggagcctac gtttttatta tcttcaccgg
     1981 cttcctcatt accttcttgg cttttacctt cttcaaagtc cctgagaccc gtggcaggac
     2041 ttttgaggat atcacacggg cctttgaagg gcaggcacac ggtgcagata gatctggaaa
     2101 ggacggcgtc atggagatga acagcatcga gcctgctaag gagaccacca ccaatgtctg
     2161 attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2221 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2281 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2341 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     2401 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     2461 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     2521 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2581 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     2641 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2701 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     2761 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2821 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2881 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2941 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     3001 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     3061 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     3121 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     3181 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     3241 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     3301 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3361 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3421 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3481 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     3541 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3601 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3661 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3721 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3781 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3841 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3901 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3961 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4021 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4081 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4141 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4201 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4261 ttgctggcct tttgctcaca tgtt
//
