LOCUS       sequence-169204-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular
            linkers, and Tev protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Hartfield RM, Schwarz KA, Muldoon JJ, Bagheri N, Leonard JN
  TITLE     Multiplexing Engineered Receptors for Multiparametric Evaluation of 
            Environmental Ligands.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2042-2055. doi: 
            10.1021/acssynbio.6b00279. Epub 2017 Aug 23.
  PUBMED    28771312
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00279"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2042-2055"
COMMENT     Sequence Label: Rap-MESA-20-Tev
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(164..868)
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="ESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKH
                     LFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQR
                     EERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIV
                     GIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEE
                     PFQPVKEATQLMN"
ORIGIN
        1 aaggaacctt taatagaaat tggacagcaa gaaagcgagc ttagtgatac ttgtgggcca
       61 gggcattagc cacaccagcc accactttct gataggcagc ctgcactggt ggggtgaatt
      121 gctcgaggtt taaacagctc caccgcggtg gcggccgcta ctaattcatg agttgagtcg
      181 cttccttaac tggctgaaaa ggctcttcag gtttcaccat gaaaacttta tggccccccc
      241 acaatactga gtcagcattt aatcgccaac cactaaccca ctgctgcgcc tcctgatttg
      301 tcaacaattc catgaagttt ttcggcacgc ttgtgaaata attgtttgtg ttggtgaaat
      361 tcgatgctga gtgtatacca acaatgaacc catctctagt tgatactaat ggactgccac
      421 actgcccatc cttggtttga atccaatgct tccagaatat gccatcagat gaagggaatg
      481 tgcaactagt gtctgacacc atgctagaca tgctcttagt ttggaagttg gttgtcacaa
      541 gacatatgcg ctcttccctt tgtggctctc taaatttcag cttttgagga aatggtggga
      601 aatccttagg catgcgaata attatcatgt ccctcccatc aatgaggtgt tgttgcaaag
      661 tcgtggtgtt cttgaccttg aatacaccat gtagtgattg gaccaacagt gttccattat
      721 ttcttctaaa caagtgcttg tttgtaatga tgaagggacc aaatccaata ccatacaacg
      781 atgttgtgtg cccatcagat tcattcgtca aatgacaaat ggtgctcgat atcgggttgt
      841 aatcacgcgg ccccttaaac aagctctcgt tttca
//
