LOCUS       sequence-169238-        6459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6459)
  TITLE     PPAR gamma
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe bleo human PPAR gamma 2
FEATURES             Location/Qualifiers
     source          1..6459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             16..485
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    550..749
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             950..1366
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1296..1318
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1334..1350
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2975..2995)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2980..3309
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3160..3295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3369..3743
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             3961..4430
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4560..4577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4731..5319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4811..4830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5479..6270,6271..6339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6102..6121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6340..6444)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agtgccacct gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
       61 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
      121 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
      181 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
      241 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
      301 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
      361 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
      421 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
      481 tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca
      541 ccgggaggca agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac
      601 tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg
      661 tggtggaact gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt
      721 tgggggccgt ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc
      781 aggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
      841 tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt
      901 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt
      961 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
     1021 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
     1081 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
     1141 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
     1201 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
     1261 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
     1321 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccagt
     1381 gtggtggtac gtaggaattc gccagcacag tggtcgagcg gaaaccccta ttccatgctg
     1441 ttatgggtga aactctggga gattctccta ttgacccaga aagcgattcc ttcactgata
     1501 cactgtctgc aaacatatca caagaaatga ccatggttga cacagagatg ccattctggc
     1561 ccaccaactt tgggatcagc tccgtggatc tctccgtaat ggaagaccac tcccactcct
     1621 ttgatatcaa gcccttcact actgttgact tctccagcat ttctactcca cattacgaag
     1681 acattccatt cacaagaaca gatccagtgg ttgcagatta caagtatgac ctgaaacttc
     1741 aagagtacca aagtgcaatc aaagtggagc ctgcatctcc accttattat tctgagaaga
     1801 ctcagctcta caataagcct catgaagagc cttccaactc cctcatggca attgaatgtc
     1861 gtgtctgtgg agataaagct tctggatttc actatggagt tcatgcttgt gaaggatgca
     1921 agggtttctt ccggagaaca atcagattga agcttatcta tgacagatgt gatcttaact
     1981 gtcggatcca caaaaaaagt agaaataaat gtcagtactg tcggtttcag aaatgccttg
     2041 cagtggggat gtctcataat gccatcaggt ttgggcggat gccacaggcc gagaaggaga
     2101 agctgttggc ggagatctcc agtgatatcg accagctgaa tccagagtcc gctgacctcc
     2161 gggccctggc aaaacatttg tatgactcat acataaagtc cttcccgctg accaaagcaa
     2221 aggcgagggc gatcttgaca ggaaagacaa cagacaaatc accattcgtt atctatgaca
     2281 tgaattcctt aatgatggga gaagataaaa tcaagttcaa acacatcacc cccctgcagg
     2341 agcagagcaa agaggtggcc atccgcatct ttcagggctg ccagtttcgc tccgtggagg
     2401 ctgtgcagga gatcacagag tatgccaaaa gcattcctgg ttttgtaaat cttgacttga
     2461 acgaccaagt aactctcctc aaatatggag tccacgagat catttacaca atgctggcct
     2521 ccttgatgaa taaagatggg gttctcatat ccgagggcca aggcttcatg acaagggagt
     2581 ttctaaagag cctgcgaaag ccttttggtg actttatgga gcccaagttt gagtttgctg
     2641 tgaagttcaa tgcactggaa ttagatgaca gcgacttggc aatatttatt gctgtcatta
     2701 ttctcagtgg agaccgccca ggtttgctga atgtgaagcc cattgaagac attcaagaca
     2761 acctgctaca agccctggag ctccagctga agctgaacca ccctgagtcc tcacagctgt
     2821 ttgccaagct gctccagaaa atgacagacc tcagacagat tgtcacggaa cacgtgcagc
     2881 tactgcaggt gatcaagaag acggagacag acatgagtct tcacccgctc ctgcaggaga
     2941 tctacaagga cttgtactag ccgctcgacc ctgtggaatg tgtgtcagtt agggtgtgga
     3001 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3061 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3121 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3181 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3241 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3301 ttttgcaaaa agcttgccag ctggggcgcc ctctggtaag gttgggaagc cctgcagatc
     3361 tgatcaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     3421 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     3481 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     3541 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     3601 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     3661 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     3721 tcgtggccga ggagcaggac tgaccgacgc cgaccaacac cgccggtccg acgcggcccg
     3781 acgggtccga ggcatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     3841 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     3901 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     3961 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     4021 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     4081 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     4141 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     4201 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     4261 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     4321 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     4381 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat
     4441 gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat
     4501 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4561 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4621 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4921 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5161 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5401 tcaaaaagga tcttcaccta gatccttttg cggccgcaaa tcaatctaaa gtatatatga
     5461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6421 aaataaacaa ataggggttc cgcgcacatt tccccgaaa
//
