LOCUS       sequence-169426-        6753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector expressing NDUFAF5-HA-IRES-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander
            ES, Sabatini DM
  TITLE     Gene Essentiality Profiling Reveals Gene Networks and Synthetic 
            Lethal Interactions with Oncogenic Ras.
  JOURNAL   Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 
            10.1016/j.cell.2017.01.013.
  PUBMED    28162770
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013."
COMMENT     Sequence Label: pMXs-NDUFAF5-HA
FEATURES             Location/Qualifiers
     source          1..6753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             23..539
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    603..960
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1019..1435
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1365..1387
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1403..1419
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1445..1819
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1757..1773
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2919..2945
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2968..2991
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(3030..3604,3605..3607,3608..3616)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3197..3214)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3424..3443
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3617..4012
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4258..4727
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4857..4874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5028..5616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5108..5127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5780..6571,6572..6640))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6403..6422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6641..6745)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      241 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      301 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      361 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga ttgactgagt
      421 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
      481 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
      541 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      601 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
      661 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
      721 actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga cttcgggggc
      781 cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt tttggactct ttggtgcacc
      841 cccctaatag gagggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
      901 tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg
      961 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
     1021 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     1081 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     1141 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     1201 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     1261 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     1321 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     1381 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat
     1441 atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga
     1501 catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt
     1561 ccagcacgaa gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt
     1621 ggtacctcac ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa
     1681 cctagaacct cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa
     1741 agtagacggc atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg
     1801 tggaccatcc tctagactgc cggatctagc tagttaatta aggatccgcc accatgctgc
     1861 ggccggcagg gctctggcgc ttatgtcggc gaccttgggc ggcgagggtc ccagcggaga
     1921 atcttggccg tagggaagtc acctctggtg tctctccccg cggtagcacc tcgcccagaa
     1981 ccctgaatat tttcgaccgg gatttgaaaa ggaaacagaa gaactgggca gcccggcagc
     2041 ccgagccgac caaatttgac tacctgaagg aggaggttgg aagtcggatc gcagaccgtg
     2101 tatatgacat acccagaaat ttcccccttg ctttggatct tggttgtgga agaggttaca
     2161 ttgcacaata tttgaataag gaaactattg gaaagttttt ccaagctgac attgcagaaa
     2221 atgctttgaa aaattcctca gaaacagaaa tacctactgt cagcgtttta gctgatgaag
     2281 aattccttcc cttcaaagaa aatacatttg acctggtggt tagcagttta agtttgcatt
     2341 gggtgaatga ccttcctaga gcacttgagc agattcatta tattttaaaa ccagatggag
     2401 tgtttatcgg tgcaatgttt ggaggcgaca cactctatga acttcggtgt tccttacagt
     2461 tagcggaaac ggaaagggaa ggaggatttt ctccacacat ttctcctttc actgctgtca
     2521 atgacctggg acatctgctt gggagagctg gctttaatac tctgactgtg gacactgatg
     2581 aaattcaagt taactatcct gggatgtttg aattgatgga agatttacaa ggtatgggtg
     2641 agagtaactg tgcttggaat agaaaagccc tgctgcatcg agacacaatg ctggcagctg
     2701 cggcagtgta cagagaaatg tacagaaatg aagatggttc agtacctgct acataccaga
     2761 tctattacat gataggatgg aaatatcatg agtcacaggc aagaccagct gaaagaggtt
     2821 ccgcaactgt gtcatttgga gagctaggaa aaataaacaa ccttatgcca ccagggaaaa
     2881 aatcacaagc ggccgcagga ggcggtggag gcggtggata cccctatgat gtgcctgact
     2941 acgcatgatt ataaagatca tgacattgat tacaaggatg acgatgacaa gtaataataa
     3001 attccgcccc cccccccccc cccccccccc ccctctccct cccccccccc taacgttact
     3061 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3121 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3181 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     3241 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     3301 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     3361 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     3421 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     3481 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     3541 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     3601 taatatggcc acaaccatgg ttcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     3661 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     3721 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     3781 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     3841 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     3901 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     3961 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagtcgacga
     4021 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     4081 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     4141 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
     4201 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
     4261 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4321 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4381 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     4441 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     4501 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     4561 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     4621 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     4681 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     4741 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     4801 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     4861 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4921 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5461 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5701 aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg
     5761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6721 aaaataaaca aataggggtt ccgcgcacat ttc
//
