LOCUS       sequence-169438-        5302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Hong H, Takahashi K, Ichisaka T, Aoi T, Kanagawa O, Nakagawa M, 
            Okita K, Yamanaka S
  TITLE     Suppression of induced pluripotent stem cell generation by the 
            p53-p21 pathway.
  JOURNAL   Nature. 2009 Aug 27. 460(7259):1132-5.
  PUBMED    19668191
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Aug 27. 460(7259):1132-5."
COMMENT     Sequence Label: pMXs-DsRed Express
FEATURES             Location/Qualifiers
     source          1..5302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..678
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(131..149)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(181..201)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     399..418
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     593..616
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     LTR             866..1335
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1465..1482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1636..2224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1716..1735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2388..3179,3180..3248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3011..3030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3249..3353)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3383..3976
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4039..4396
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4455..4871
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4801..4823
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4839..4855
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    4881..5255
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5193..5209
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      join(5297..5302,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
       61 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      121 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      181 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
      241 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      301 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggctc cttcatctac
      361 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca gaagaagact
      421 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
      481 atccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
      541 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggactc caagctggac
      601 atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcgc cgagggccgc
      661 caccacctgt tcctgtagcg gccgccagca cagtggtcga cgataaaata aaagatttta
      721 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
      781 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
      841 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
      901 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
      961 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     1021 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     1081 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     1141 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     1201 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     1261 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     1321 agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat
     1381 ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1441 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1501 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1561 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1621 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1681 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1741 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1801 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1861 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1921 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1981 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2041 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2101 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2161 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2221 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2281 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc
     2341 ggccgcaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2401 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2461 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2521 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2581 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2641 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2701 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2761 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2821 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2881 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2941 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3001 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3061 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3361 ccccgaaaag tgccacctgc ataatgaaag accccacctg taggtttggc aagctagctt
     3421 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
     3481 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     3541 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     3601 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     3661 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     3721 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     3781 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
     3841 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     3901 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     3961 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
     4021 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
     4081 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg
     4141 gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg ggagacgtcc
     4201 cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc gatcgttttg
     4261 gactctttgg tgcacccccc taataggagg gatatgtggt tctggtagga gacgagaacc
     4321 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga agccgcgccg
     4381 cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     4441 tgtctgaaaa ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga
     4501 aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta
     4561 ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta
     4621 accgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
     4681 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
     4741 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
     4801 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
     4861 ctctaggcgc ccccatatgg ccatatgaga tcttatatgg ggcacccccg ccccttgtaa
     4921 acttccctga ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac
     4981 aggctctcta cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac
     5041 aactggaccg accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc
     5101 gacaccagac taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca
     5161 cccccaccgc cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg
     5221 ctgccgaccc cgggggtgga ccatcctcta gactgccgga tctagctagt taattaagga
     5281 tccccgggta ccggtcgcca cc
//
