LOCUS       sequence-169496-       11856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrate plasmid of Streptococcus pneumoniae, for chromosome 
            integration of IPTG-inducible dCas9sp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Liu X, Gallay C, Kjos M, Domenech A, Slager J, van Kessel SP, Knoops
            K, Sorg RA, Zhang JR, Veening JW
  TITLE     High-throughput CRISPRi phenotyping identifies new essential genes 
            in Streptococcus pneumoniae.
  JOURNAL   Mol Syst Biol. 2017 May 10;13(5):931. doi: 10.15252/msb.20167449.
  PUBMED    28490437
REFERENCE   2  (bases 1 to 11856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2017-05-10"; volume: "13(5):931. doi"; pages: " 
            10.15252/msb.20167449"
COMMENT     Sequence Label: pJWV102-PL-dCas9
FEATURES             Location/Qualifiers
     source          1..11856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(267..1058,1059..1127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     890..909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1128..1232)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      5398..5469
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5485..5512
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    5609..5628
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     RBS             5664..5675
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5682..5708,5709..5711,5712..8198,8199..8201,
                     8202..9788)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(10930..10952)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11038..11178
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11093..11112
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11193..11210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11364..11856,1..96))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11444..11463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
       61 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
      541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
      601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
      661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
      901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aagaattaat tccttcttaa cgccccaagt tcatcaccaa tgacatcaac
     1381 tcacatgaac tacatgatga acccagttat catggttttg gataagattt ttgaaaaatt
     1441 cttcccaggc cttgataaat atgactttga tgctgctaaa ttgaacaaga aaatcggttt
     1501 ctggggatct aaattcttca tcggtttcat ccttggtatc gttatcggta ttatgggaac
     1561 tccacatcca attgcaggtg ttgcagatgc agataaatgg cgtcttgtta tcaaaggatg
     1621 gttgtctctt ggtttgactg ccggtgtatc tttggaactc ttctcactta tcggttcatg
     1681 gttcatcgca gccgtagaac cactatcaca aggtattaca aacgttgcta ctaaacgtct
     1741 tcaaggacgt aaattcaata tcggtcttga ctggccattc atcgctggtc gtgctgaaat
     1801 ctgggcttgt gccaacgtac ttgcaccaat catgttgatt gaagcagtgc ttctttcaaa
     1861 agttggaaat ggtatcttgc cacttgcagg tatcatcgct atgggtgtta ctccagctct
     1921 cttggttgta actcgtggta aattgctccg tatgattatc ttcggaacac tcttgttgcc
     1981 actcttcctt ctttcaggta cacttattgc accatttgca acagaacttg ctaaaggtgt
     2041 aggtgccttc ccagaaggtg tgagccaaac tcaattgatt actcactcta ctcttgaagg
     2101 accaatcgaa aaacttcttg gttggacaat tggtaacact acaactggtg atatcaaagc
     2161 aatccttggt gcagtagcct tccttgtatt ctatatcggt atctttgctt ggtacagaaa
     2221 acaaatgatc aaacgtaacg aagagtacgc agcaaaagca aaataatgcg ctccgctagc
     2281 tttacagaca aagaactatc cttaatggaa gcactagcaa aaggtatcaa tgaagcaaga
     2341 aatattgaag gctagtcagt aaaattcaga taaacaaaaa gagccgataa gatgagagtt
     2401 tcatttctca aattatcggc tctgcgtctt ggcgtctggc tctttgattg ttattatatt
     2461 cattttttga acattaatta aagtttcgtt cttacatttg gggcagtata aagggaaatt
     2521 tttaagtata gtatccactc gtattcgtag tcttgtttta tttccacaaa taggacacaa
     2581 tatccacttg tagtttataa taacaatctc ctcctttcca ctttaattca aatctatatt
     2641 aaagaatatt tcatcttatt taataagaaa ccatatttat ataacaacat aaaacgcact
     2701 aagttatttt attgaacata tatctcactt tatctatctg actatttaga cgacgggtct
     2761 ggcaaacagg ttcgccagtg gtaacctgat atccttttag ctctgctaaa caaacactaa
     2821 gcccatttgt aaaaaaagtt aaatcattgc gataatcttg aatacatcga gcaggaattt
     2881 ctccaataat aatgacctca ttatttttca gttgagtatt tacgatattt gcacaatatt
     2941 tgggagcatc gttatatgcc cgtgaaagat attcctgtgg tgcataaact ttaaaactaa
     3001 gatatggctc taacaattct gttccagctt ttctaaaggc ttgctccaat acaataggag
     3061 caagcatccg aaaatctgct ggggtactaa cagggctata gtataagcca tacttaaaac
     3121 agattttaca gtccgtcaca ttccaaccat acaatccttg ttcacaacca tagcgtatcc
     3181 cttccataac tgcattttga aatgattgat ttaagtatcc aagagaaacc gagctctcat
     3241 actgcattcc acttcccaac ggaagcggtg atacagataa accaatggaa gcccagaaag
     3301 gatttggcgg cacttcgatg tgaatggtat attctgcatt ttttaacggt ctctccatat
     3361 aaatgactgt aggctctttt agttctatct ccacatgata cttttcttgc aacagtgcac
     3421 taatcacttc catttgtact ttccctaaga aagaaagtat aatttcatgt gtcgtagaat
     3481 ccacgtaata tcgtagaagc ggatcactat ctgagatttc caaaagggca tcaagcaaca
     3541 tttctctctg ttcaggttta ctcggttcaa cagttgtttg tagtagaggg tgcggatttt
     3601 caatcttttt tctctgtggc aatagttttg tatctccaag aacactattt aacttcaaaa
     3661 gctcattttg caaaataaca atttctccag aataagctcg atcaatctta cataattcac
     3721 catttattga agtatacatt tctgtaactt ttattttttc tttttctgat actctaaccg
     3781 aatctcgtaa atgtagtact ccactataaa ggcgtatata tgcaagacgt tgtctttttt
     3841 ttgtatattc aattttgaaa acatttccgc aaagttcaga cggacctcga tgtgttgatg
     3901 aataaaattt attagtaata acttctataa ggttatcaat ccctatatta ctttttgcac
     3961 ttccatgata aagagggaac agagaacaat tctgaaatct tatgctttcc tcttgttcga
     4021 gttccaatgc ttctaatgat ttaccggaca tatatttctc taaaaggtca tcgtttccct
     4081 ctattaccgt atcccattgt tcagattcgg taaagttcgt cacacacaca ttaggataca
     4141 gttctacctt ctgtttgatt acaatttcgg cagaaagttt ctctttaata tcctgataaa
     4201 ccgttgataa atcaattcca ttttggtcaa tcttattgat aaaaaagatt gtgggaatcc
     4261 ccattttcct aagtgcatga aataatatac gagtttgtgc ttgtacgcca tcttttgcag
     4321 aaatcagtag aattgcccca tctaaaactg ataatgaacg atatacttct gctaagaaat
     4381 ccatatgtcc tggcgtgtct atgatgttca ccttcgtatt ttcccactga aaagaggtta
     4441 ttcctgtctg aattgtaatt cctctctgac gttctaaaag cgtattatcc gtcctcgttg
     4501 tacctttgtc cacgcttcct aattctgtaa tcgctccact gttatataat aagctttctg
     4561 ttaaggtagt ttttcctgca tcaacatgag ctaaaactcc aatattaata attttcatgt
     4621 gattttcctc cattcaaaag cccaaaaggg cataaaaatc ccagtgataa atactcttat
     4681 cactgggatt tttatgcata accataggca tacaaagcat acagatattc tccggatact
     4741 ttagaatcac atgataaagg tattcttaaa ctggggacaa aaaactaagc cctcctaaaa
     4801 aaggacgtcc aattatttgt tcccgctatc aaattgacag tttatttaag aataccttgc
     4861 cgcatattta ttaactcctt ttaaatagat acttaaatta tagcacgtaa gagcatattt
     4921 gtaaaggaat ctccaatttt ttatcaaaaa gagtacatga ttacaaagta tctgtaatca
     4981 tgtaccaata tttattattt tacaatcttc caattatctt gtttttctaa tatcaactca
     5041 aattgcgaga tttgggttgc ctttgtttct tgatctaaat atttcacagc gacattcacg
     5101 attacttggc tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca
     5161 ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc
     5221 gactacgcga tcatggcgac cacacccgtc ctgtggatct atcgatgcat gcagccggta
     5281 ccatcacaag cactttggga cgttctccct tagtgctttt ttgatttctc ataggccggc
     5341 ctgttagtca tatggacact taagctctag acggtgatca acacgctagc caggcatcaa
     5401 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     5461 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttataga
     5521 attcactagt aattgtgagg cggataacaa ttctcactca ttctacagtt tattcttgac
     5581 attgcactgt ccccctggta taataactaa ttgtgagcgc tcacaattaa gatccccggg
     5641 gcggccgcga attcaaaaga tctaaagagg agaaaggatc tatggataag aaatactcaa
     5701 taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat gaatataagg
     5761 ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc
     5821 ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt ctcaaacgga
     5881 cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag gagatttttt
     5941 caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag tcttttttgg
     6001 tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta gatgaagttg
     6061 cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta gattctactg
     6121 ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag tttcgtggtc
     6181 attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa ctatttatcc
     6241 agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca agtggagtag
     6301 atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa aatctcattg
     6361 ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct ttgtcattgg
     6421 gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa ttacagcttt
     6481 caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga gatcaatatg
     6541 ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca gatatcctaa
     6601 gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa cgctacgatg
     6661 aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt ccagaaaagt
     6721 ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt gatgggggag
     6781 ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg gatggtactg
     6841 aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg acctttgaca
     6901 acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag
     6961 aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc
     7021 gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca tggatgactc
     7081 ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt
     7141 cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag
     7201 tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa ttgacaaagg
     7261 tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag
     7321 ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag
     7381 attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat
     7441 ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat aaagattttt
     7501 tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg accttatttg
     7561 aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt gatgataagg
     7621 tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct cgaaaattga
     7681 ttaatggtat tagggataag caatctggca aaacaatatt agattttttg aaatcagatg
     7741 gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca tttaaagaag
     7801 acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat attgcaaatt
     7861 tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat
     7921 tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg gcacgtgaaa
     7981 atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag
     8041 gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat actcaattgc
     8101 aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat gtggaccaag
     8161 aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca caaagtttcc
     8221 ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat cgtggtaaat
     8281 cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg agacaacttc
     8341 taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct gaacgtggag
     8401 gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa actcgccaaa
     8461 tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac gatgaaaatg
     8521 ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt tctgacttcc
     8581 gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat gcccatgatg
     8641 cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa cttgaatcgg
     8701 agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct aagtctgagc
     8761 aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg aacttcttca
     8821 aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc gaaactaatg
     8881 gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg cgcaaagtat
     8941 tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc ggattctcca
     9001 aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa aaagactggg
     9061 atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc ctagtggttg
     9121 ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta ctagggatca
     9181 caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa gctaaaggat
     9241 ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt tttgagttag
     9301 aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga aatgagctgg
     9361 ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa aagttgaagg
     9421 gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag cattatttag
     9481 atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca gatgccaatt
     9541 tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt gaacaagcag
     9601 aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct tttaaatatt
     9661 ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta gatgccactc
     9721 ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt cagctaggag
     9781 gtgactaagt cgacctcgag actagtcaag gtcggcaatt ctgcagtact aggacgccgc
     9841 caagccagct taaacccagc tcaatgagct gggttttttg tttgttaaaa atgaagaaga
     9901 aactgtgaag cgtatttata gcaaagcact caaaagttta ccttatgggt gcttttttcg
     9961 tgcttttttg aaaagacaaa aaaaagaacc ttgccaagca agattctttt gatagcgcta
    10021 tcgctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
    10081 ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttctggtga
    10141 tgaaatcaac gtaacattta aagctgtcaa agccaaagtc atgagatggc gtatggagcg
    10201 taaagctgac aagagcggtg ttgcgatgat tgagatgacc ttccttgcac caagtgaatt
    10261 gcctcaagaa agcactcaat caaagattct tgtagatgga aaagaacttg ctgatttcgc
    10321 tgaaaatcgt caagactatc aaattaccta taaaggtcaa cggccaaaag tctcagttga
    10381 agaaaacaat caagtagctt caactgtggt agatagtgga gaagatagcc ttccagtact
    10441 tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa taccgtatcc agttgactaa
    10501 ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa ctcgaatttg ttgaaaaaga
    10561 tttggcctac aagacagttg agaaaaaaga ttcaacactg tatctaggtg aaactcgtgt
    10621 agaacaagaa ggaaaagttg gaaaagaacg tatctttaca gtgattaatc ctgatggaag
    10681 taaggaagaa aaactccgtg aagtggtaga agttccgaca gaccgcatcg tcttggttgg
    10741 aaccaaacca gtagctcaag aagctaaaaa accacaagtg tcagaaaaag cagatacaaa
    10801 accaattgat tcaagtgaag ctgatcaaac taataaagcc cagttaccaa atacaggtag
    10861 tgcggcaagc caagcagcag tagcagcagg tttagcctgc ctcgcgcgtt tcggtgatga
    10921 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10981 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    11041 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
    11101 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    11161 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11761 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    11821 tgcgctctgc tgaagccagt taccttcgga aaaaga
//
