LOCUS       sequence-169602-        7987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SOBIR1-mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Postma J, Liebrand TW, Bi G, Evrard A, Bye RR, Mbengue M, Kuhn H, 
            Joosten MH, Robatzek S
  TITLE     Avr4 promotes Cf-4 receptor-like protein association with the 
            BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and
            plant immunity.
  JOURNAL   New Phytol. 2016 Apr;210(2):627-42. doi: 10.1111/nph.13802. Epub 
            2016 Jan 14.
  PUBMED    26765243
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.13802";
            journalName: "New Phytol"; date: "2016-04"; volume: "210"; issue: 
            "2"; pages: "627-42"
COMMENT     Sequence Label: p35S::AtSOBIR1-mCherry
FEATURES             Location/Qualifiers
     source          1..7987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1940..1964)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1972..2682
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2117..2135)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2385..2404
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2975..2999
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3111..3820
                     /label=oriV
                     /note="incP origin of replication"
     promoter        3893..3984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3985..4053,4054..4845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4203..4222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5016..5604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5505..5524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    5916..5940
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6034..6286)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6306..6857)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(6899..7078)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        7547..7891
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7835..7855
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     7893..7909
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7894..7910
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7943..7967
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggctgttc ccacgggaag cgccaatctc tttctccggc ctctcatcct cgccgttctc
       61 tcctttcttc ttctctcttc attcgtctcc tctgttgagt ggctcgatat cgattcctca
      121 gatctcaaag ctcttcaggt catcgaaaca gagctcggag tcaacagtca acgttcttcg
      181 gccagtgatg tcaacccttg cggtcgccga ggagttttct gcgagagacg gcactctgcc
      241 accaccggag aatacgttct ccgtgtcacg aggctcgtct accgatccag gagcttgacg
      301 gggactatct ctccggtcat cggaatgttg tcggagctca aggaacttac tttgtctaat
      361 aaccagttgg tcaacgctgt tccggtggat atcttaagct gtaagcaact tgaagttctt
      421 gatcttcgga aaaatagatt ttccggtcag attccgggaa acttttctag tttgagccgt
      481 cttcgaatcc ttgatctctc ttcgaacaag ttatccggga acttgaattt cttgaagaat
      541 ctacgcaatc tggagaatct ctctgttgca aacaatctct tctcaggcaa aatcccggaa
      601 caaattgtgt cgttccacaa tctccggttc tttgatttct ccggaaaccg gtatttggaa
      661 ggtccggctc cggttatgag cagtatcaag cttcagacat ctccacatca aacaagacac
      721 attcttgctg aaactccaac ttcaagtccc acgaacaaac ccaacaacag cactacatca
      781 aaagctccaa agggagctcc aaaaccaggg aagttgaaga agaagaagaa gaagagcaag
      841 aagaagaaag tagcggcatg gatcttaggg tttgtcgttg gagccatagg aggaacaatc
      901 tctgggtttg tcttctcggt gttgttcaaa ttgattattc aagcaataag aggatcagaa
      961 aaaccaccag gtccttccat attcagccca ttgatcaaga aagctgaaga tttagctttc
     1021 ttggagaatg aagaagcgtt agcttctctg gagatcatag gaagaggagg ttgtggagaa
     1081 gttttcaaag ctgaactacc aggaagcaat gggaagatca tagctgtgaa gaaagtgatc
     1141 caaccgccta aagacgccga tgaactaaca gatgaagatt ccaagtttct gaacaagaaa
     1201 atgaggcaaa ttagatccga gatcaacaca gtcggccata tccggcaccg gaatcttctc
     1261 ccattgttag cacacgtgtc aagacccgag tgccactacc tcgtttacga gtacatggaa
     1321 aaggggagtt tgcaagatat attgactgac gtacaagctg gaaatcaaga actaatgtgg
     1381 ccagcgaggc acaagattgc tttaggtata gctgcagggc ttgagtacct tcacatggat
     1441 cataacccac gaatcattca cagagactta aagccagcca atgttcttct tgatgatgac
     1501 atggaagcca gaatttcaga tttcggactg gcaaaggcaa tgccagatgc agtcacacat
     1561 attacaacct cgcatgttgc aggtactgtg ggatacatag caccagagtt ttatcaaacc
     1621 cacaagttca cagataaatg tgatatctat agttttggag tgattcttgg gattctggtg
     1681 attgggaagc ttccttcaga tgagtttttt cagcatactg acgagatgag tctgataaag
     1741 tggatgagga acataataac atcggaaaat cctagccttg cgattgatcc gaagttgatg
     1801 gatcaaggat tcgatgaaca gatgcttctg gttctgaaga tcgcctgtta ctgtactttg
     1861 gatgatccaa aacagaggcc gaacagtaaa gatgtcagga ccatgttgtc ccagatcaag
     1921 cacaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttgatgg gatggtgagc
     1981 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     2041 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     2101 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     2161 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     2221 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2281 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     2341 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     2401 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     2461 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     2521 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     2581 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     2641 ggccgccact ccaccggcgg catggacgag ctgtacaagt aagagctcta gagtccgcaa
     2701 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
     2761 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
     2821 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     2881 tcctaaaacc aaaatccagt gaccgggcgg ccgccaccgc ggtggagggg gatcagattg
     2941 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     3001 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     3061 ccgttcgtcc atttgtatgt gtacatcacc gacgagcaag gcaagaccga gcgcctttcc
     3121 gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa
     3181 cgcgccagaa acgccgtcga agccgtgtgc gagacaccgg ccgccggcgt tgtggatacc
     3241 tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg
     3301 actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga
     3361 gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga
     3421 tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg
     3481 acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc
     3541 acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc
     3601 gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa
     3661 accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg
     3721 cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc ccaggggctg
     3781 cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt ccttgccatt
     3841 gccgggatcg gggcagtaac gggatgggcg atcagcccga aagctacccc tatttgttta
     3901 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3961 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4021 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4081 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4141 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4201 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4261 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4321 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4381 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4441 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4501 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4561 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4621 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4681 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4741 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4801 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4861 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4921 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4981 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5041 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5101 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5161 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5221 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5281 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5341 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5401 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5461 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5521 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5581 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5641 ttttgctggc cttttgctca catggactct agctagagga tcacaggcag caacgctctg
     5701 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     5761 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     5821 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     5881 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     5941 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgat cgaatactaa
     6001 cgtctctacc agatatcagc ttgcatgccg gtcgatctag taacatagat gacaccgcgc
     6061 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
     6121 attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa
     6181 ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa
     6241 caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct tgactctagg
     6301 ggtcatcaga tttcggtgac gggcaggacc ggacggggcg gcaccggcag gctgaagtcc
     6361 agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg
     6421 ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg
     6481 atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag
     6541 agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt cgactcggcc
     6601 gtccagtcgt aggcgttgcg tgccttccag ggacccgcgt aggcgatgcc ggcgacctcg
     6661 ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc
     6721 cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg
     6781 ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg
     6841 cgtcgttctg ggctcatggt agatcccctc gatcgagttg agagtgaata tgagactcta
     6901 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     6961 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     7021 cttagctcat taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg
     7081 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     7141 cttaattctc cgctcatgat cagattgtcg tttcccgcct tcggtttggg cgcgcccggt
     7201 tcagaagacc agagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
     7261 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc
     7321 ttctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctaccgac
     7381 agtggtccca aagatggacc cccacccacg aggaacatcg tggaaaaaga agacgttcca
     7441 accacgtctt caaagcaagt ggattgatgt gatacatggt ggagcacgac actctcgtct
     7501 actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac
     7561 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     7621 aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg
     7681 ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga
     7741 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     7801 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     7861 tataaggaag ttcatttcat ttggagagga cctcgaggtc gacggtatcg ataagcttga
     7921 tatcgaattc ctgcagccct caacaagttt gtacaaaaaa gcaggctccg cggccgcccc
     7981 cttcacc
//
