LOCUS       sequence-169604-        6063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Talin Head Domain, H, Excitation:604, Emission:659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6063)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCardinal-Talin-H-18
FEATURES             Location/Qualifiers
     source          1..6063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             249..980
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     regulatory      1029..1038
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2487..2608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2524..2543)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2578..2597
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2615..3070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2752..2773)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2964..2983
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3097..3201
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3203..3560
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3203..3394
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3226..3246)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3411..3546
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3595..4389
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3595..4389
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3649..3668)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4259..4278
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4577..4596)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4621..4668
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4997..5585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5486..5505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5760..6063
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgatcaagga gaacatgcac atgaagctgt
      301 acatggaagg caccgtgaac aaccaccact tcaagtgcac caccgaaggg gagggcaagc
      361 cctacgaggg cacccagacc cagaggatta aggtggtgga gggaggcccc ctgccgttcg
      421 cattcgacat cctggccacc tgctttatgt acgggagcaa gaccttcatc aaccacaccc
      481 agggcatccc cgatttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca
      541 ccacatacga agacgggggc gtgcttaccg ttacccagga caccagcctc caggacggct
      601 gcttgatcta caacgtcaag ctcagagggg tgaacttccc atccaacggc cctgtgatgc
      661 agaagaaaac actcggctgg gaggccacca ccgagaccct gtaccccgct gacggcggcc
      721 tggaaggcag atgcgacatg gccctgaagc tcgtgggcgg gggccacctg cactgcaacc
      781 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact
      841 ttgtggaccg cagactggaa agaatcaagg aggccgacaa tgagacctac gtcgagcagc
      901 acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatg
      961 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagctagc ttcgaattcg
     1021 cagaagctgc caccatggtt gcgctttcgc tgaagattag cattgggaat gtggtgaaga
     1081 cgatgcaatt tgagccatct accatggtgt acgacgcctg ccgcatgatt cgtgagcgga
     1141 tcccagaggc cctggctggc cctcccaacg actttgggct ctttctgtca gatgatgacc
     1201 ccaaaaaagg catctggcta gaggctggga aagctttgga ctactacatg ctccgaaatg
     1261 gggacaccat ggagtacaga aaaaagcaga gacccctgaa gatccggatg ttagacggaa
     1321 cggtgaagac tatcatggtg gatgactcca agaccgtcac agacatgctc atgaccattt
     1381 gtgcccgaat tggtatcacc aaccatgatg agtattcact ggttcgagag ctgatggaag
     1441 aaaagaaaga tgaggggaca gggaccctga gaaaagacaa gaccctactg cgagatgaaa
     1501 agaagatgga gaagctaaag cagaagttgc acacagacga tgagttgaac tggctggacc
     1561 atgggcggac actgagggaa cagggagtgg aagagcatga gacgttgctg ctgcggagaa
     1621 agttcttcta ctcagaccag aatgtggatt cccgagaccc tgtacagctg aaccttctct
     1681 atgtgcaggc acgagatgac atcctgaatg gctcccatcc tgtctccttt gacaaggcct
     1741 gtgaatttgc aggcttccag tgccagatcc agtttggacc tcacaatgaa cagaagcaca
     1801 aggctggctt ccttgacctg aaggacttcc tgcccaagga gtatgtgaag cagaagggag
     1861 agcgtaagat ctttcaggca cacaagaatt gtgggcagat gagtgaaatt gaggccaagg
     1921 tacgctacgt gaagctggcc cgttccctca agacttacgg tgtctccttc ttcctagtca
     1981 aggaaaagat gaaggggaag aataaactgg tgcccaggct gttgggcatc actaaggagt
     2041 gtgtgatgcg tgtggatgag aagaccaagg aggtgattca ggaatggagc ctcaccaaca
     2101 tcaaacgctg ggctgcctct cccaagagct ttactctgga ctttggagac tatcaggatg
     2161 gctactactc agtacagacg acagaaggcg agcagatcgc acagctcatt gctggctaca
     2221 tagatatcat ccttaagaag aaaaaaagca aggaccattt tgggctggag ggagatgaag
     2281 agtctactat gttggaggac tcagtgtctc ccaaaaagtc aacagtcctt cagtaagtcg
     2341 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
     2401 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2461 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     2521 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2581 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta
     2641 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     2701 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     2761 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     2821 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     2881 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     2941 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3001 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3061 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3121 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3181 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     3241 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3301 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3361 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3421 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3481 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3541 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     3601 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     3661 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     3721 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     3781 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     3841 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     3901 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     3961 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4021 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4081 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4141 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4201 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4261 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4321 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4381 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     4441 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     4501 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     4561 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4621 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     4681 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     4741 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     4801 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     4861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
     5701 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     5761 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     5821 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     5881 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     5941 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     6001 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     6061 atg
//
