LOCUS       sequence-169682-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 in fission yeast using fluoride selection and targetting
            pil1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Fernandez R, Berro J
  TITLE     Use of a fluoride channel as a new selection marker for fission 
            yeast plasmids and application to fast genome editing with 
            CRISPR/Cas9.
  JOURNAL   Yeast. 2016 Oct;33(10):549-557. doi: 10.1002/yea.3178. Epub 2016 Sep
            7.
  PUBMED    27327046
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.3178"; 
            journalName: "Yeast"; date: "2016-10"; volume: "33"; issue: "10"; 
            pages: "549-557"
COMMENT     Sequence Label: pJB172
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctattaa cacagtctta cttttgtata atgtctatgt tggggacgct agctcgtcta
       61 ggacttactg cattaaacac atacccaggt gcaccgtttt ctggactttt atgggttcaa
      121 tttgttggat gcgttattat gggattttgt cagacagagt ctgttttctt tcctcgaccc
      181 aaacataatg ctactttttt gctagccatt actacaggtt tttgtggatc tcttacaacg
      241 ttttcttctt ggatgttgca aatgtttaca gggatggcta acttggatcc atttgaacgt
      301 aggggccgag gctattcatt tttaagtgtg gtttccgatt ttatggtaac tatgtgcatt
      361 gcgatgtcca gcttaatatg ggggaagcag attggaaaaa cgacgggaca gtggagaatt
      421 ggcaaagttg catttgcctg gcctatcccg gcgcacacgc atatcgtagt gcgggtatta
      481 cttttgttac tctcgatttg tttttttgtt ggtgcagcat tttatacggc atatacaacc
      541 aatgtgacac atcgtggcat aggttttagt ctaatattct ctccttttgc tgccttgaca
      601 cgtttgtatc ttgcgcgatt ccttaattca cctcaatatt ttattcccta tggaactctt
      661 tgtgccaatg tctttgctac acttttgctt tctatcatgt acatgatacc acaaatcaca
      721 cactgcactc ctgtgtctag aagtgtgatg tatggaatcc aaaacggatt ttgtgctgta
      781 ctctctaccc tttcaacatt ctccaacgaa ctccacacaa tgcctataaa acgagcatat
      841 atttattgta taatttcagt tgctatttcc ttttcaatat gtgtcattgt cgatggagct
      901 actgcatggg gtcatggtta tactgaaaag tattag
//
