LOCUS       sequence-169773-        5160 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Quantitation Reporter (PQR) to quantify a protein of 
            interest in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE
  TITLE     Quantification of Protein Levels in Single Living Cells.
  JOURNAL   Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 
            10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10.
  PUBMED    26686644
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.11.048"; journalName: "Cell Rep"; date: 
            "2015-12-22- 22"; volume: "13"; issue: "11"; pages: "2634-44"
COMMENT     Sequence Label: pCAG-GFP-PQRv3-RFP
FEATURES             Location/Qualifiers
     source          1..5160
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..72
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     134..153
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      187..196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             931..987
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             988..1719
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             1756..1785
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1876..1895)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1941..1996
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1995..2014)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2357..2373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2357..2373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2370..2392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2381..2397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2405..2411,2412..2429,2430..2435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2450..2471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2530..2725
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2576..2711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2731..2865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2768..2787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2822..2841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2933..2950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3104..3692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3184..3203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3863..4654,4655..4723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4486..4505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4724..4828)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
       61 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
      121 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
      181 tgagccgcca ccatggccaa tttaatggtg tccaaaggag aagaactgtt cactggcgtc
      241 gtgcccattc tggtggagct cgacggggat gtgaacgggc ataaatttag tgtgagaggg
      301 gagggcgagg gcgatgccac taacgggaag cttaccctca agttcatttg taccaccggc
      361 aagcttcctg tcccctggcc taccctcgtc accacactca catacggcgt gcagtgcttt
      421 tccaggtacc cagaccacat gaagcggcac gacttcttta agtccgccat gcccgaggga
      481 tacgtgcagg agcggaccat cagctttaag gatgacggga catacaagac aagagccgag
      541 gtgaagttcg agggggacac tctggtgaac cggattgagc tgaaggggat tgattttaag
      601 gaggatggga acatcctggg ccacaagctg gagtacaact ttaactccca taacgtgtac
      661 atcaccgccg acaagcagaa gaacgggatc aaagccaact tcaagatccg gcacaacgtc
      721 gaagatggca gcgtccagct ggccgatcac taccagcaaa acacaccaat tggggacgga
      781 cccgtgctgt tgcccgacaa ccactacttg agcacacaga gcgtcttgag caaagaccca
      841 aacgagaaga gggatcacat ggtcttgaag gagagggtga ctgccgctgg gattacacac
      901 ggcatggacg agctctataa gggaagcgga gcgacgaatt ttagtctact gaaacaagcg
      961 ggagacgtgg aggaaaaccc tggacctatg gtgtctaagg gcgaagagct gattaaggag
     1021 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     1081 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
     1141 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga
     1201 accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc
     1261 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     1321 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1381 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac cgagatgctg
     1441 taccccgctg acggcggcct ggaaggcaga accgacatgg ccctgaagct cgtgggcggg
     1501 ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc taagaacctc
     1561 aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga ggccgacaaa
     1621 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1681 ctggggcaca aacttaatgg catggacgag ctgtacaagt gataacgcct gctggaagat
     1741 gaagatggac cggtggaaca aaaacttatt tctgaagaag atctgtgata gcggccgcac
     1801 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     1861 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     1921 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     1981 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     2041 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     2101 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     2161 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     2221 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     2281 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     2341 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2401 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2461 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2521 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     2581 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2641 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     2701 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     2761 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2821 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     2881 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3541 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3841 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3901 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3961 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4021 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4081 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4141 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4201 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4261 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4321 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4381 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4441 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4501 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4561 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4621 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4681 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4741 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4801 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     4861 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4921 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4981 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     5041 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     5101 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
//
